|
Status |
Public on Jan 21, 2021 |
Title |
WT-L3-3 |
Sample type |
SRA |
|
|
Source name |
rabbits liver tissue
|
Organism |
Oryctolagus cuniculus |
Characteristics |
tissue: liver disease state: rabbit model of hereditary tyrosinemia type I (HT1)
|
Extracted molecule |
total RNA |
Extraction protocol |
The livers of treated rabbits' were trypsinized and collected, and total mRNA was extracted using TRIzol reagent The TruSeq RNA Sample Preparation kit (Illumina) was used to construct RNA-seq libraries
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
High-throughput sequencing was performed on the llumina NovaSeq . Trimmed reads were obtained by removing adapter sequences, and reads with low complexity or of low quality were removed. quality filtered reads were mapped to the rabbit reference genome using Salmon 0.14.1 Genome_build: oryCun2 Supplementary_files_format_and_content: comma-delimited csv files include Raw reads counts of each Sample ...
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|
|
Submission date |
Jan 21, 2021 |
Last update date |
Jan 22, 2021 |
Contact name |
Shixue Gou |
Organization name |
GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH,CHINESE ACADEMY OF SCIENCES
|
Street address |
No. 190, Kaiyuan Avenue, Huangpu District, Guangzhou
|
City |
Guangzhou |
ZIP/Postal code |
510530 |
Country |
China |
|
|
Platform ID |
GPL26786 |
Series (2) |
GSE142722 |
CRISPR/Cas9-mediated gene correction in newborn rabbits with hereditary tyrosinemia type I |
GSE142782 |
CRISPR/Cas9-mediated gene correction in newborn rabbits with hereditary tyrosinemia type I [RNA-Seq] |
|
Relations |
BioSample |
SAMN17442088 |
SRA |
SRX9908701 |