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Status |
Public on Jan 01, 2012 |
Title |
CD11b+Gr1+ cells from the lung of tumor challenged mice rep2 |
Sample type |
RNA |
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Source name |
Lung of tumor challenged mice
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Organism |
Mus musculus |
Characteristics |
days: 9 days tissue: Lung genotype: wild-type cell type: CD11b+Gr1+
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Growth protocol |
Mice were challenged with LLC-RFP cells (5×10^6 cells, subcutaneous injection) for 9 days. Single cell suspensions were prepared from lungs of both tumor challenged and control wild type mice and stained with antibodies against CD11b and Gr1. Approximately 1×10^5 CD11b+GR1+ myeloid progenitor cells were sorted by FACS (Aria II, BD Bioscience).
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from sorted CD11b+Gr1+ cells by using Picopure RNA Isolation kit (Arcturus)
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Label |
biotin
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Label protocol |
RNA quality was measured using Agilent’s RNA 6000 Pico LabChip. Complementary DNA was synthesized and amplified with WT-Ovation™ Pico RNA Amplification System (NuGene Technologies Inc), followed by sense transcript cDNA generation, fragmentation and labeling with WT-Ovation™ Exon Module and FL-Ovation™ cDNA Biotin Module V2 (NuGene Technologies Inc).
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Hybridization protocol |
Labeled samples were hybridized 16 hours at 45° at 60rpm, followed by washing and staining with streptavidin-phycoerythrin in the Affymetrix Fluidics Station 450
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Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
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Description |
CD11b+Gr1+ cells from the lung of tumor challenged mice
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Data processing |
Probe level analysis was performed using robust multi array average (RMA) normalization, and data analysis was performed using ArrayAssist software package (Stratagene) probe group file: MoEx-1_0-st-v1.r2.pgf meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm8.mps
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Submission date |
Feb 03, 2010 |
Last update date |
Jan 01, 2012 |
Contact name |
Dingcheng Gao |
E-mail(s) |
dig2009@med.cornell.edu
|
Organization name |
Weill Cornell Medical College
|
Department |
CT Surgery
|
Street address |
1300 York Ave
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
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Platform ID |
GPL6096 |
Series (1) |
GSE20180 |
Expression data of Sorted CD11b+Gr1+ cells from mice with or without LLC tumor challenging |
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