|
| Status |
Public on Jan 01, 2012 |
| Title |
CD11b+Gr1+ cells from the lung of control wild type mice rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Lung of control wild type mice
|
| Organism |
Mus musculus |
| Characteristics |
days: N/A
tissue: Lung
genotype: wild-type
cell type: CD11b+Gr1+
|
| Growth protocol |
Mice were challenged with LLC-RFP cells (5×10^6 cells, subcutaneous injection) for 9 days. Single cell suspensions were prepared from lungs of both tumor challenged and control wild type mice and stained with antibodies against CD11b and Gr1. Approximately 1×10^5 CD11b+GR1+ myeloid progenitor cells were sorted by FACS (Aria II, BD Bioscience).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from sorted CD11b+Gr1+ cells by using Picopure RNA Isolation kit (Arcturus)
|
| Label |
biotin
|
| Label protocol |
RNA quality was measured using Agilent’s RNA 6000 Pico LabChip. Complementary DNA was synthesized and amplified with WT-Ovation™ Pico RNA Amplification System (NuGene Technologies Inc), followed by sense transcript cDNA generation, fragmentation and labeling with WT-Ovation™ Exon Module and FL-Ovation™ cDNA Biotin Module V2 (NuGene Technologies Inc).
|
| |
|
| Hybridization protocol |
Labeled samples were hybridized 16 hours at 45° at 60rpm, followed by washing and staining with streptavidin-phycoerythrin in the Affymetrix Fluidics Station 450
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
CD11b+Gr1+ cells from the lung of control wild type mice
|
| Data processing |
Probe level analysis was performed using robust multi array average (RMA) normalization, and data analysis was performed using ArrayAssist software package (Stratagene)
probe group file: MoEx-1_0-st-v1.r2.pgf
meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm8.mps
|
| |
|
| Submission date |
Feb 03, 2010 |
| Last update date |
Jan 01, 2012 |
| Contact name |
Dingcheng Gao |
| E-mail(s) |
dig2009@med.cornell.edu
|
| Organization name |
Weill Cornell Medical College
|
| Department |
CT Surgery
|
| Street address |
1300 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE20180 |
Expression data of Sorted CD11b+Gr1+ cells from mice with or without LLC tumor challenging |
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