|
| Status |
Public on Feb 13, 2010 |
| Title |
midguts fed BSA + Ecoli rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Anopheles gambiae midguts 3hr after a BSA + heat killed E. coli meal
|
| Organism |
Anopheles gambiae |
| Characteristics |
tissue: midgut
genotype: G3 strain
age: 5 to 6 day-old mosquitoes
|
| Treatment protocol |
Anopheles gambiae G3 mosquitoes 5-6 day-old were fed BSA (20% in PBS with fresh 10 mM sodium bicarbonate) with or without heat killed E. coli (equivalent of 2.5 ml of 0.8 OD) . Three pools of 10 mosquito midguts were dissected after 3h and processed for microarray analysis of gene expression.
|
| Growth protocol |
Anopheles gambiae mosquitoes strain G3 were reared at 27C and 80% humidity on a 12-hr light dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the RNeasy Mini kit (Qiagen) following manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix GeneChip Expression 3'-Amplification, One-cycle target labeling assay from 5 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 48 hr at 45C on GeneChip Plasmodium falciparum Anopheles gambiae Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 7Gplus GeneChip scanner.
|
| Description |
Gene expression data from midguts 3hr after a artifitial protein meal containing heat killed E. coli.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Feb 04, 2010 |
| Last update date |
Feb 12, 2010 |
| Contact name |
Alvaro Molina-Cruz |
| E-mail(s) |
amolina-cruz@niaid.nih.gov
|
| Phone |
301-435-2706
|
| Fax |
301-480-0930
|
| Organization name |
NIH
|
| Department |
NIAID
|
| Lab |
LMVR
|
| Street address |
12735 Twinbrook Parkway
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20852 |
| Country |
USA |
| |
|
| Platform ID |
GPL1321 |
| Series (1) |
| GSE20204 |
Expression data from the mosquito Anopheles gambiae midgut 3h after a protein meal with or without E. coli. |
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