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Sample GSM508528 Query DataSets for GSM508528
Status Public on Feb 02, 2011
Title 5 Ko
Sample type RNA
 
Source name LSK cell from Gfi1 knockout mice
Organism Mus musculus
Characteristics cell type: LSK
tissue: hematopoietic stem cell enriched fraction from bone marrow
Treatment protocol LSKs were sorted by FACS
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Rneasy mini kit (QIAGEN)
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the artus ExpressArt mRNA 2-rouind amplification protocol (nano version) from 10 ng total RNA, using the ENZO kit for labelling in the second IVT reaction
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Arrays were scanned in a GeneArray Scanner (Agilent).
Description Gene expression data from LSK cell from Gfi1 knockout mice
Data processing The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Feb 11, 2010
Last update date Feb 02, 2011
Contact name Ludger Klein-Hitpass
E-mail(s) ludger.klein-hitpass@uni-essen.de
Phone +49 201 723 85552
Organization name Institut fuer Zellbiologie
Department Universitaetsklinikum
Lab BioChip Lab
Street address Virchowstr. 173
City Essen
ZIP/Postal code D-45122
Country Germany
 
Platform ID GPL339
Series (1)
GSE20282 Gfi1 regulates survival and lineage commitment of hematopoietic precursors and prevents myeloproliferative diseases

Data table header descriptions
ID_REF
VALUE MAS5 Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 402.513 P 0.0103167
AFFX-BioB-M_at 729.504 P 8.14279e-05
AFFX-BioB-3_at 273.605 P 0.00844047
AFFX-BioC-5_at 1246.45 P 0.000169227
AFFX-BioC-3_at 672.552 P 8.14279e-05
AFFX-BioDn-5_at 660.055 P 4.42873e-05
AFFX-BioDn-3_at 5454.8 P 0.000258358
AFFX-CreX-5_at 10064.1 P 4.42873e-05
AFFX-CreX-3_at 16456.1 P 4.42873e-05
AFFX-DapX-5_at 83.5923 M 0.0629293
AFFX-DapX-M_at 59.2764 A 0.185131
AFFX-DapX-3_at 7.09462 A 0.963431
AFFX-LysX-5_at 8.65793 A 0.783476
AFFX-LysX-M_at 54.3755 A 0.544587
AFFX-LysX-3_at 22.9915 A 0.205732
AFFX-PheX-5_at 8.68246 A 0.916408
AFFX-PheX-M_at 12.6116 A 0.686277
AFFX-PheX-3_at 45.3565 A 0.559354
AFFX-ThrX-5_at 19.575 A 0.737173
AFFX-ThrX-M_at 67.9505 A 0.262827

Total number of rows: 22690

Table truncated, full table size 698 Kbytes.




Supplementary file Size Download File type/resource
GSM508528.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM508528.CHP.gz 6.0 Mb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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