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Sample GSM508529 Query DataSets for GSM508529
Status Public on Feb 02, 2011
Title 7 Ko
Sample type RNA
 
Source name LSK cell from Gfi1 knockout mice
Organism Mus musculus
Characteristics cell type: LSK
tissue: hematopoietic stem cell enriched fraction from bone marrow
Treatment protocol LSKs were sorted by FACS
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Rneasy mini kit (QIAGEN)
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the artus ExpressArt mRNA 2-rouind amplification protocol (nano version) from 10 ng total RNA, using the ENZO kit for labelling in the second IVT reaction
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Arrays were scanned in a GeneArray Scanner (Agilent).
Description Gene expression data from LSK cell from Gfi1 knockout mice
Data processing The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Feb 11, 2010
Last update date Feb 02, 2011
Contact name Ludger Klein-Hitpass
E-mail(s) ludger.klein-hitpass@uni-essen.de
Phone +49 201 723 85552
Organization name Institut fuer Zellbiologie
Department Universitaetsklinikum
Lab BioChip Lab
Street address Virchowstr. 173
City Essen
ZIP/Postal code D-45122
Country Germany
 
Platform ID GPL339
Series (1)
GSE20282 Gfi1 regulates survival and lineage commitment of hematopoietic precursors and prevents myeloproliferative diseases

Data table header descriptions
ID_REF
VALUE MAS5 Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 297.135 P 0.0284573
AFFX-BioB-M_at 540.921 P 0.000340305
AFFX-BioB-3_at 199.272 P 0.0151826
AFFX-BioC-5_at 766.777 P 0.000224668
AFFX-BioC-3_at 397.779 P 0.000581214
AFFX-BioDn-5_at 536.848 P 8.14279e-05
AFFX-BioDn-3_at 3391.5 P 0.000856509
AFFX-CreX-5_at 6584.69 P 4.42873e-05
AFFX-CreX-3_at 11350.6 P 4.42873e-05
AFFX-DapX-5_at 59.8968 A 0.250796
AFFX-DapX-M_at 62.43 A 0.147939
AFFX-DapX-3_at 4.36567 A 0.986189
AFFX-LysX-5_at 40.2818 A 0.5
AFFX-LysX-M_at 80.5974 A 0.5
AFFX-LysX-3_at 38.0857 A 0.275146
AFFX-PheX-5_at 4.65899 A 0.937071
AFFX-PheX-M_at 13.9291 A 0.617401
AFFX-PheX-3_at 19.9022 A 0.794268
AFFX-ThrX-5_at 20.149 A 0.724854
AFFX-ThrX-M_at 51.5839 A 0.41138

Total number of rows: 22690

Table truncated, full table size 698 Kbytes.




Supplementary file Size Download File type/resource
GSM508529.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM508529.CHP.gz 6.1 Mb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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