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Sample GSM508530 Query DataSets for GSM508530
Status Public on Feb 02, 2011
Title 8 Ko
Sample type RNA
 
Source name LSK cell from Gfi1 knockout mice
Organism Mus musculus
Characteristics cell type: LSK
tissue: hematopoietic stem cell enriched fraction from bone marrow
Treatment protocol LSKs were sorted by FACS
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Rneasy mini kit (QIAGEN)
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the artus ExpressArt mRNA 2-rouind amplification protocol (nano version) from 10 ng total RNA, using the ENZO kit for labelling in the second IVT reaction
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Arrays were scanned in a GeneArray Scanner (Agilent).
Description Gene expression data from LSK cell from Gfi1 knockout mice
Data processing The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Feb 11, 2010
Last update date Feb 02, 2011
Contact name Ludger Klein-Hitpass
E-mail(s) ludger.klein-hitpass@uni-essen.de
Phone +49 201 723 85552
Organization name Institut fuer Zellbiologie
Department Universitaetsklinikum
Lab BioChip Lab
Street address Virchowstr. 173
City Essen
ZIP/Postal code D-45122
Country Germany
 
Platform ID GPL339
Series (1)
GSE20282 Gfi1 regulates survival and lineage commitment of hematopoietic precursors and prevents myeloproliferative diseases

Data table header descriptions
ID_REF
VALUE MAS5 Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 304.562 P 0.00359458
AFFX-BioB-M_at 608.619 P 0.000258358
AFFX-BioB-3_at 227.555 P 0.000662269
AFFX-BioC-5_at 885.353 P 0.000126798
AFFX-BioC-3_at 485.922 P 5.16732e-05
AFFX-BioDn-5_at 546.967 P 4.42873e-05
AFFX-BioDn-3_at 4444.48 P 0.000146581
AFFX-CreX-5_at 8610.33 P 4.42873e-05
AFFX-CreX-3_at 14061.3 P 4.42873e-05
AFFX-DapX-5_at 38.7666 A 0.195266
AFFX-DapX-M_at 45.5661 A 0.165861
AFFX-DapX-3_at 32.3292 A 0.672921
AFFX-LysX-5_at 43.3917 A 0.156732
AFFX-LysX-M_at 11.2725 A 0.712257
AFFX-LysX-3_at 58.509 P 0.0429619
AFFX-PheX-5_at 5.62235 A 0.9273
AFFX-PheX-M_at 5.5624 A 0.794268
AFFX-PheX-3_at 56.9073 A 0.48511
AFFX-ThrX-5_at 11.0423 A 0.794268
AFFX-ThrX-M_at 3.86364 A 0.834139

Total number of rows: 22690

Table truncated, full table size 701 Kbytes.




Supplementary file Size Download File type/resource
GSM508530.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM508530.CHP.gz 6.1 Mb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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