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Sample GSM508531 Query DataSets for GSM508531
Status Public on Feb 02, 2011
Title 5 wt
Sample type RNA
Source name LSK cell from Gfi1 wildtype mice
Organism Mus musculus
Characteristics cell type: LSK
tissue: hematopoietic stem cell enriched fraction from bone marrow
Treatment protocol LSKs were sorted by FACS
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Rneasy mini kit (QIAGEN)
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the artus ExpressArt mRNA 2-rouind amplification protocol (nano version) from 10 ng total RNA, using the ENZO kit for labelling in the second IVT reaction
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Arrays were scanned in a GeneArray Scanner (Agilent).
Description Gene expression data from LSK cell from Gfi1 wildtype mice
Data processing The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling as normalization method.
Submission date Feb 11, 2010
Last update date Feb 02, 2011
Contact name Ludger Klein-Hitpass
Phone +49 201 723 85552
Organization name Institut fuer Zellbiologie
Department Universitaetsklinikum
Lab BioChip Lab
Street address Virchowstr. 173
City Essen
ZIP/Postal code D-45122
Country Germany
Platform ID GPL339
Series (1)
GSE20282 Gfi1 regulates survival and lineage commitment of hematopoietic precursors and prevents myeloproliferative diseases

Data table header descriptions
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)

Data table
AFFX-BioB-5_at 538.335 P 0.002867
AFFX-BioB-M_at 789.91 P 0.000169227
AFFX-BioB-3_at 330.652 P 0.0020226
AFFX-BioC-5_at 1365.52 P 0.00010954
AFFX-BioC-3_at 703.416 P 0.000195116
AFFX-BioDn-5_at 633.603 P 5.16732e-05
AFFX-BioDn-3_at 6169.98 P 0.000195116
AFFX-CreX-5_at 12119.4 P 4.42873e-05
AFFX-CreX-3_at 18514.3 P 4.42873e-05
AFFX-DapX-5_at 45.1067 A 0.48511
AFFX-DapX-M_at 76.2807 A 0.131361
AFFX-DapX-3_at 9.1491 A 0.9273
AFFX-LysX-5_at 19.8305 A 0.724854
AFFX-LysX-M_at 30.5349 A 0.51489
AFFX-LysX-3_at 97.2246 M 0.0542134
AFFX-PheX-5_at 7.4392 A 0.932322
AFFX-PheX-M_at 33.1937 A 0.645547
AFFX-PheX-3_at 61.3102 A 0.631562
AFFX-ThrX-5_at 85.0046 A 0.617401
AFFX-ThrX-M_at 96.001 A 0.123572

Total number of rows: 22690

Table truncated, full table size 698 Kbytes.

Supplementary file Size Download File type/resource
GSM508531.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM508531.CHP.gz 6.0 Mb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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