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Sample GSM508533 Query DataSets for GSM508533
Status Public on Feb 02, 2011
Title 8 wt
Sample type RNA
Source name LSK cell from Gfi1 wildtype mice
Organism Mus musculus
Characteristics cell type: LSK
tissue: hematopoietic stem cell enriched fraction from bone marrow
Treatment protocol LSKs were sorted by FACS
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Rneasy mini kit (QIAGEN)
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the artus ExpressArt mRNA 2-rouind amplification protocol (nano version) from 10 ng total RNA, using the ENZO kit for labelling in the second IVT reaction
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Arrays were scanned in a GeneArray Scanner (Agilent).
Description Gene expression data from LSK cell from Gfi1 wildtype mice
Data processing The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling as normalization method.
Submission date Feb 11, 2010
Last update date Feb 02, 2011
Contact name Ludger Klein-Hitpass
Phone +49 201 723 85552
Organization name Institut fuer Zellbiologie
Department Universitaetsklinikum
Lab BioChip Lab
Street address Virchowstr. 173
City Essen
ZIP/Postal code D-45122
Country Germany
Platform ID GPL339
Series (1)
GSE20282 Gfi1 regulates survival and lineage commitment of hematopoietic precursors and prevents myeloproliferative diseases

Data table header descriptions
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)

Data table
AFFX-BioB-5_at 469.549 P 0.00499819
AFFX-BioB-M_at 638.753 P 0.00010954
AFFX-BioB-3_at 319.428 P 0.0020226
AFFX-BioC-5_at 1298.78 P 0.000126798
AFFX-BioC-3_at 746.402 P 6.02111e-05
AFFX-BioDn-5_at 727.539 P 4.42873e-05
AFFX-BioDn-3_at 5440.56 P 0.000146581
AFFX-CreX-5_at 12427.9 P 4.42873e-05
AFFX-CreX-3_at 20013.1 P 4.42873e-05
AFFX-DapX-5_at 28.804 A 0.354453
AFFX-DapX-M_at 46.0498 A 0.165861
AFFX-DapX-3_at 6.46156 A 0.987453
AFFX-LysX-5_at 4.09857 A 0.814869
AFFX-LysX-M_at 17.7726 A 0.617401
AFFX-LysX-3_at 39.3643 A 0.262827
AFFX-PheX-5_at 5.27119 A 0.852061
AFFX-PheX-M_at 10.9748 A 0.672921
AFFX-PheX-3_at 38.7476 A 0.52976
AFFX-ThrX-5_at 7.77203 A 0.891021
AFFX-ThrX-M_at 31.4408 A 0.5

Total number of rows: 22690

Table truncated, full table size 701 Kbytes.

Supplementary file Size Download File type/resource
GSM508533.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM508533.CHP.gz 6.0 Mb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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