|
| Status |
Public on Jun 02, 2021 |
| Title |
CD73- AD-MSCs of mice rep3 |
| Sample type |
RNA |
| |
|
| Source name |
CD73 negative adipose derived mesenchymal stem cells of the C57BL/6 mice
|
| Organism |
Mus musculus |
| Characteristics |
cell type: AD-MSCs were isolated from adipose tissues surrounding the groin of mice.
|
| Treatment protocol |
The same number of cells were digested and centrifuged, and then the cells were collected in RNA enzyme free Ep tubes.
|
| Growth protocol |
Cells were cultured with low sugar DMEM(Dulbecco’s modified Eagle’s medium) containing 10% FBS (Fetal bovine serum; Hyclone, USA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using Trizol. The RNA quantity and quality were measured by NanoDrop 2000.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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| |
|
| Hybridization protocol |
Following fragmentation, 10ug of cRNA were hybridized for 16 hr at 45C on PrimeView Human Gene Expression Array. GeneChips were washed and stained in the GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 .
|
| Description |
CD73- AD-MSCs were isolated using flow cytometry and then cultured.
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method.
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| |
|
| Submission date |
Feb 22, 2021 |
| Last update date |
Jun 02, 2021 |
| Contact name |
Huifang Hou |
| E-mail(s) |
liangshuang1979@xxmu.edu.cn
|
| Phone |
+86 18637359227
|
| Organization name |
Xinxiang Medical University
|
| Street address |
601 Jinsui Avenue
|
| City |
Xinxiang |
| ZIP/Postal code |
453003 |
| Country |
China |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE167219 |
Expression data from CD73 positive adipose derived mesenchymal stem cells (CD73+ AD-MSCs) and CD73 negative adipose derived mesenchymal stem cells (CD73- AD-MSCs) of mouse |
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