|
| Status |
Public on Nov 16, 2021 |
| Title |
Splenic B cells, WT_B_3 |
| Sample type |
RNA |
| |
|
| Source name |
Splenic B cells from WT B6 mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Spleen
cell type: B cells
strain: C57BL/6
genotype: WT
|
| Treatment protocol |
Splenic B cells sort-purifed from WT or Zeb2 -165-/- mice.
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Column extraction of total RNA was performed acording to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the Affymetrix WT Pico reagent kit from 100pg-2ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation and labeling, 2.5 ug of cDNA were hybridized onto the Gene 1.0 ST arrays and placed in GeneChip Hybridization Oven 640 for 18 hr at 45C. Gene Chip’s were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip 7G 3000 Scanner.
|
| Description |
Splenic B cells from WT B6 mouse
|
| Data processing |
The data was analyzed with the Affymetrix GeneChip Command Console. Microarray expression data was processed using Command Console (Affymetrix, Inc) and the raw (.CEL) files generated were analyzed using Expression Console software with Affymetrix default RMA Gene analysis settings.(Affymetrix, Inc). Probe summarization (Robust Multichip Analysis, RMA), quality control analysis, and probe annotation were performed according to recommended guidelines (Expression Console Software, Affymetrix, Inc.).
|
| |
|
| Submission date |
Feb 24, 2021 |
| Last update date |
Nov 16, 2021 |
| Contact name |
Xiao Huang |
| E-mail(s) |
huangxiao@wustl.edu
|
| Organization name |
Washington University in St. Louis, School of Medicine
|
| Department |
Pathology and Immunology
|
| Lab |
Kenneth Murphy
|
| Street address |
4940 Parkview Place, CSRB 7766, Department of Pathology and Immunology
|
| City |
St. Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE167458 |
Expression data from mouse splenic B cells |
|
