|
| Status |
Public on Jul 01, 2010 |
| Title |
WT-T2 |
| Sample type |
RNA |
| |
|
| Source name |
WT colon tumor tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: wild type
tissue: colon
disease state: tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
Standard Illumina protocol
|
| Description |
Replicate of sample 5
|
| Data processing |
The raw data (see Supplementary file 'GSE20407_matrix_non-normalized.txt' on Series record) were obtained from Beadstudio version 3.2.7.
The raw data were normalized using Genesifter software. Specifically, the raw intensity of each gene on the Illumina microarray was log transformed and normalized according to the total mean of the whole microarray (hence, normalized "all mean" values).
|
| |
|
| Submission date |
Feb 18, 2010 |
| Last update date |
Feb 18, 2010 |
| Contact name |
Garabet Yeretssian |
| Organization name |
McGill
|
| Department |
Medicine
|
| Lab |
Saleh
|
| Street address |
3649 Promenade Sir-William Osler
|
| City |
Montreal |
| State/province |
QC |
| ZIP/Postal code |
H3G 0B1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE20407 |
Control of intestinal homeostasis, colitis and colitis-associated colorectal cancer by the inflammatory caspases |
|
