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Sample GSM512116 Query DataSets for GSM512116
Status Public on Oct 02, 2010
Title submandibular gland wild type rep3A
Sample type RNA
 
Source name mouse submandibular gland
Organism Mus musculus
Characteristics strain: C57BL/6
gender: female
age: 2 months
tissue: submandibular gland
genotype: wild type
Extracted molecule total RNA
Extraction protocol Total RNAs were purified using the RNeasy Mini Kit (Qiagen, Basel, Switzerland). On-column DNase digestion was performed during RNA purification process. RNA concentrations, A260/280, and A260/230 ratios were determined using the ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, USA).
Label Biotin
Label protocol Using Illumina TotalPrep RNA Amplification kit (#IL1791, Ambion), following the manufacturer's protocol. Input amount: 300ng of total RNA. Incubation of labeling reaction: 16h.
 
Hybridization protocol 750 ng of each sample was hybridized to Illumina´s Sentrix® MouseRef-8 v2 BeadChip, at 58 degree for 18 h
Scan protocol Chips were scanned with Illumina BeadArray Reader v.3.5.49.29917 (Factor=1.52, PMT=552, Filter=100%)
Description n/a
Data processing Array data were normalized with Chipster version 1.3.0 using the quantile normalization method
 
Submission date Feb 19, 2010
Last update date Oct 02, 2010
Contact name Pei-wen Pan
Organization name Institute of Medical Technology, University of Tampere
Street address Biokatu 6
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL6885
Series (1)
GSE20423 Gene expression profiling in the submandibular gland, stomach, and duodenum of CAVI-deficient mice

Data table header descriptions
ID_REF
VALUE quantile normalization
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 5.81 0.5037594
ILMN_3122480 5.73 0.7368421
ILMN_2599935 7.06 0
ILMN_2675543 6 0.1303258
ILMN_2686883 5.87 0.3558897
ILMN_2751818 5.77 0.6466165
ILMN_2728634 6.01 0.1190476
ILMN_3040515 6.07 0.0689223
ILMN_2711608 6.84 0
ILMN_1232875 5.86 0.3809524
ILMN_1258507 5.77 0.6265664
ILMN_2746142 5.71 0.8120301
ILMN_1252690 6.02 0.1077694
ILMN_2655499 5.85 0.4072682
ILMN_1252870 5.99 0.1466165
ILMN_1248179 5.81 0.5100251
ILMN_2649955 6.36 0.001253133
ILMN_2628708 5.78 0.5989975
ILMN_3024781 5.89 0.2957394
ILMN_2705628 5.95 0.1904762

Total number of rows: 25697

Table truncated, full table size 636 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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