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Sample GSM512119 Query DataSets for GSM512119
Status Public on Oct 02, 2010
Title stomach wild type rep2B
Sample type RNA
 
Source name mouse stomach
Organism Mus musculus
Characteristics strain: C57BL/6
gender: female
age: 2 months
tissue: stomach
genotype: wild type
Extracted molecule total RNA
Extraction protocol Total RNAs were purified using the RNeasy Mini Kit (Qiagen, Basel, Switzerland). On-column DNase digestion was performed during RNA purification process. RNA concentrations, A260/280, and A260/230 ratios were determined using the ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, USA).
Label Biotin
Label protocol Using Illumina TotalPrep RNA Amplification kit (#IL1791, Ambion), following the manufacturer's protocol. Input amount: 300ng of total RNA. Incubation of labeling reaction: 16h.
 
Hybridization protocol 750 ng of each sample was hybridized to Illumina´s Sentrix® MouseRef-8 v2 BeadChip, at 58 degree for 18 h
Scan protocol Chips were scanned with Illumina BeadArray Reader v.3.5.49.29917 (Factor=1.52, PMT=552, Filter=100%)
Description n/a
Data processing Array data were normalized with Chipster version 1.3.0 using the quantile normalization method
 
Submission date Feb 19, 2010
Last update date Oct 02, 2010
Contact name Pei-wen Pan
Organization name Institute of Medical Technology, University of Tampere
Street address Biokatu 6
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL6885
Series (1)
GSE20423 Gene expression profiling in the submandibular gland, stomach, and duodenum of CAVI-deficient mice

Data table header descriptions
ID_REF
VALUE quantile normalization
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 6.13 0.2030075
ILMN_3122480 6.09 0.2568922
ILMN_2599935 8 0
ILMN_2675543 6.15 0.1754386
ILMN_2686883 6.41 0.03258145
ILMN_2751818 6.15 0.1754386
ILMN_2728634 7.1 0
ILMN_3040515 6.31 0.05764411
ILMN_2711608 6.06 0.3057644
ILMN_1232875 6.18 0.1403509
ILMN_1258507 5.9 0.6390978
ILMN_2746142 5.65 0.9824561
ILMN_1252690 6.39 0.03634085
ILMN_2655499 5.9 0.6203008
ILMN_1252870 6.16 0.1629073
ILMN_1248179 6.45 0.02882206
ILMN_2649955 7.69 0
ILMN_2628708 6.5 0.02005013
ILMN_3024781 5.93 0.5689223
ILMN_2705628 5.97 0.4724311

Total number of rows: 25697

Table truncated, full table size 637 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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