Total RNAs were purified using the RNeasy Mini Kit (Qiagen, Basel, Switzerland). On-column DNase digestion was performed during RNA purification process. RNA concentrations, A260/280, and A260/230 ratios were determined using the ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, USA).
Using Illumina TotalPrep RNA Amplification kit (#IL1791, Ambion), following the manufacturer's protocol. Input amount: 300ng of total RNA. Incubation of labeling reaction: 16h.
750 ng of each sample was hybridized to Illumina´s Sentrix® MouseRef-8 v2 BeadChip, at 58 degree for 18 h
Chips were scanned with Illumina BeadArray Reader v.126.96.36.199917 (Factor=1.52, PMT=552, Filter=100%)
Array data were normalized with Chipster version 1.3.0 using the quantile normalization method
Feb 19, 2010
Last update date
Oct 02, 2010
Institute of Medical Technology, University of Tampere