GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM512121 Query DataSets for GSM512121
Status Public on Oct 02, 2010
Title stomach knockout rep2B
Sample type RNA
Source name mouse stomach
Organism Mus musculus
Characteristics strain: C57BL/6
gender: female
age: 2 months
tissue: stomach
genotype: Car6 -/-
Extracted molecule total RNA
Extraction protocol Total RNAs were purified using the RNeasy Mini Kit (Qiagen, Basel, Switzerland). On-column DNase digestion was performed during RNA purification process. RNA concentrations, A260/280, and A260/230 ratios were determined using the ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, USA).
Label Biotin
Label protocol Using Illumina TotalPrep RNA Amplification kit (#IL1791, Ambion), following the manufacturer's protocol. Input amount: 300ng of total RNA. Incubation of labeling reaction: 16h.
Hybridization protocol 750 ng of each sample was hybridized to Illumina´s Sentrix® MouseRef-8 v2 BeadChip, at 58 degree for 18 h
Scan protocol Chips were scanned with Illumina BeadArray Reader v. (Factor=1.52, PMT=552, Filter=100%)
Description n/a
Data processing Array data were normalized with Chipster version 1.3.0 using the quantile normalization method
Submission date Feb 19, 2010
Last update date Oct 02, 2010
Contact name Pei-wen Pan
Organization name Institute of Medical Technology, University of Tampere
Street address Biokatu 6
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL6885
Series (1)
GSE20423 Gene expression profiling in the submandibular gland, stomach, and duodenum of CAVI-deficient mice

Data table header descriptions
VALUE quantile normalization
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 6.36 0.04761905
ILMN_3122480 5.94 0.5426065
ILMN_2599935 8.1 0
ILMN_2675543 5.95 0.508772
ILMN_2686883 6.61 0.01253133
ILMN_2751818 5.8 0.8433584
ILMN_2728634 7.24 0
ILMN_3040515 6.28 0.07142857
ILMN_2711608 6.25 0.08145364
ILMN_1232875 5.96 0.4887218
ILMN_1258507 5.9 0.6240602
ILMN_2746142 5.97 0.4699248
ILMN_1252690 6.5 0.02380952
ILMN_2655499 5.95 0.508772
ILMN_1252870 6.13 0.1867168
ILMN_1248179 6.49 0.02631579
ILMN_2649955 7.82 0
ILMN_2628708 6.59 0.01253133
ILMN_3024781 5.93 0.5814536
ILMN_2705628 5.98 0.4511278

Total number of rows: 25697

Table truncated, full table size 640 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap