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Sample GSM512124 Query DataSets for GSM512124
Status Public on Oct 02, 2010
Title duodenum wild type rep1C
Sample type RNA
 
Source name mouse duodenum
Organism Mus musculus
Characteristics strain: C57BL/6
gender: female
age: 2 months
tissue: duodenum
genotype: wild type
Extracted molecule total RNA
Extraction protocol Total RNAs were purified using the RNeasy Mini Kit (Qiagen, Basel, Switzerland). On-column DNase digestion was performed during RNA purification process. RNA concentrations, A260/280, and A260/230 ratios were determined using the ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, USA).
Label Biotin
Label protocol Using Illumina TotalPrep RNA Amplification kit (#IL1791, Ambion), following the manufacturer's protocol. Input amount: 300ng of total RNA. Incubation of labeling reaction: 16h.
 
Hybridization protocol 750 ng of each sample was hybridized to Illumina´s Sentrix® MouseRef-8 v2 BeadChip, at 58 degree for 18 h
Scan protocol Chips were scanned with Illumina BeadArray Reader v.3.5.49.29917 (Factor=1.52, PMT=552, Filter=100%)
Description n/a
Data processing Array data were normalized with Chipster version 1.3.0 using the quantile normalization method
 
Submission date Feb 19, 2010
Last update date Oct 02, 2010
Contact name Pei-wen Pan
Organization name Institute of Medical Technology, University of Tampere
Street address Biokatu 6
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL6885
Series (1)
GSE20423 Gene expression profiling in the submandibular gland, stomach, and duodenum of CAVI-deficient mice

Data table header descriptions
ID_REF
VALUE quantile normalization
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 6.12 0.2719298
ILMN_3122480 6.05 0.3847118
ILMN_2599935 8.16 0
ILMN_2675543 5.9 0.6992481
ILMN_2686883 6.29 0.112782
ILMN_2751818 5.94 0.6177945
ILMN_2728634 6.98 0.006265664
ILMN_3040515 6.36 0.07894737
ILMN_2711608 7.15 0.005012531
ILMN_1232875 6.02 0.4360902
ILMN_1258507 6.19 0.179198
ILMN_2746142 5.86 0.7719298
ILMN_1252690 6.65 0.02255639
ILMN_2655499 5.95 0.5839599
ILMN_1252870 5.74 0.9323308
ILMN_1248179 6.86 0.00877193
ILMN_2649955 7.16 0.005012531
ILMN_2628708 6.35 0.08897243
ILMN_3024781 6.05 0.3834586
ILMN_2705628 6.1 0.3082707

Total number of rows: 25697

Table truncated, full table size 644 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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