NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM512126 Query DataSets for GSM512126
Status Public on Oct 02, 2010
Title duodenum knockout rep1C
Sample type RNA
 
Source name mouse duodenum
Organism Mus musculus
Characteristics strain: C57BL/6
gender: female
age: 2 months
tissue: duodenum
genotype: Car6 -/-
Extracted molecule total RNA
Extraction protocol Total RNAs were purified using the RNeasy Mini Kit (Qiagen, Basel, Switzerland). On-column DNase digestion was performed during RNA purification process. RNA concentrations, A260/280, and A260/230 ratios were determined using the ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, USA).
Label Biotin
Label protocol Using Illumina TotalPrep RNA Amplification kit (#IL1791, Ambion), following the manufacturer's protocol. Input amount: 300ng of total RNA. Incubation of labeling reaction: 16h.
 
Hybridization protocol 750 ng of each sample was hybridized to Illumina´s Sentrix® MouseRef-8 v2 BeadChip, at 58 degree for 18 h
Scan protocol Chips were scanned with Illumina BeadArray Reader v.3.5.49.29917 (Factor=1.52, PMT=552, Filter=100%)
Description n/a
Data processing Array data were normalized with Chipster version 1.3.0 using the quantile normalization method
 
Submission date Feb 19, 2010
Last update date Oct 02, 2010
Contact name Pei-wen Pan
Organization name Institute of Medical Technology, University of Tampere
Street address Biokatu 6
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL6885
Series (1)
GSE20423 Gene expression profiling in the submandibular gland, stomach, and duodenum of CAVI-deficient mice

Data table header descriptions
ID_REF
VALUE quantile normalization
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 6.32 0.07894737
ILMN_3122480 6.02 0.3984962
ILMN_2599935 8.23 0
ILMN_2675543 5.91 0.6516291
ILMN_2686883 6.23 0.131579
ILMN_2751818 6.06 0.3333333
ILMN_2728634 6.87 0.006265664
ILMN_3040515 6.21 0.1390978
ILMN_2711608 7.18 0.001253133
ILMN_1232875 6.14 0.2042606
ILMN_1258507 5.75 0.9010025
ILMN_2746142 5.72 0.9423559
ILMN_1252690 6.44 0.03759398
ILMN_2655499 5.92 0.6353384
ILMN_1252870 6.16 0.1917293
ILMN_1248179 6.29 0.09774436
ILMN_2649955 7.22 0.001253133
ILMN_2628708 7.04 0.003759399
ILMN_3024781 5.99 0.4724311
ILMN_2705628 5.81 0.8370928

Total number of rows: 25697

Table truncated, full table size 642 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap