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Sample GSM5128 Query DataSets for GSM5128
Status Public on Mar 27, 2003
Title Dendritic Cells, Exposed to Leishmania major - Repeat #1
Sample type RNA
 
Source name Monocyte-derived Dendritic Cells, Exposed to the intracellular protozan parasite Leishmania major for 18 hours
Organism Homo sapiens
Extracted molecule total RNA
 
Description CD14+ peripheral blood-derived monocytes were isolated from leukopacks from healthy donors by counterflow centrifugal elutriation. Fresh monocytes were cultured in 6-well tissue culture plates at 2-3 ยด 106/ml in complete RPMI 1640 supplemented with 20 mM glutamine, 2% heat-inactivated human AB serum, 100 ug/ml penicillin, and 100 g/ml streptomycin. For generation of DC, recombinant human (rh) IL-4 and rhGMCSF were added to the culture at 50 ng/ml on days 1, 3, and 5 of culture. Cells were exposed to the pathogens on day 8 of culture, and 16 hours later were harvested and used for RNA preparation.
Because of donor-to-donor differences, human experimental systems can be prone to considerable variability. Therefore cells from a total of 7 donors were prepared and infected independently. The RNA from all 7 donors was extracted separately; equivalent amount of RNA from each donor was pooled, and used for subsequent labeling and hybridization. We assessed the variability associated with the preparation of targets and processing of microarrays by replicating this procedure with another set of RNA from the same donors.
Keywords = Infectious diseases, Parasites, Infection, Innate immunity
 
Submission date Mar 26, 2003
Last update date Oct 28, 2014
Contact name Damien Chaussabel
E-mail(s) DChaussabel@benaroyaresearch.org
Organization name Baylor Institute for Immunology Research
Street address 3434 Live Oak
City Dallas
State/province TX
ZIP/Postal code 75204
Country USA
 
Platform ID GPL8300
Series (1)
GSE360 Macrophages and dendritic cells exposed to phylogenetically distinct parasites

Data table header descriptions
ID_REF
VALUE Average Difference intensity
ABS_CALL Present / Absent Call
F_CHANGE Fold change in gene expression compared to baseline value (untreated cells)

Data table
ID_REF VALUE ABS_CALL F_CHANGE
100_g_at 247.7 P -1.3
1000_at 79.4 P 1.1
1001_at 50.5 A 1.5
1002_f_at 12.2 A 1.2
1003_s_at -64.8 A -2.1
1004_at -18.4 A -2
1005_at 430.3 P 6.2
1006_at 294.8 P 6
1007_s_at 5.4 A -2
1008_f_at 1458.7 P 1.3
1009_at 1751.5 P 1.1
101_at 15 A -1.4
1010_at 61.4 A 1.6
1011_s_at 122.9 P 1.5
1012_at -3.5 A 1.2
1013_at 25.2 P 1.4
1014_at 135.9 P -1.4
1015_s_at 37 A -1.3
1016_s_at -3.8 A 1.1
1017_at 10.5 A -1.4

Total number of rows: 12625

Table truncated, full table size 256 Kbytes.




Supplementary data files not provided

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