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Sample GSM515392 Query DataSets for GSM515392
Status Public on Feb 24, 2010
Title WT_Chow_rep3
Sample type RNA
 
Source name mouse islets
Organism Mus musculus
Characteristics gender: male
strain: C57Bl/6Hsd
age: 10 months
tissue: islets
genotype/variation: WT
growth protocol: chow (control)
Treatment protocol To isolate the islets from pancreas, pancreas were perfused with collagenase solution.
Growth protocol Mouse were fed chow and a western diet for 6 months. The diet regimen started when they were at 4 month old
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed using a 1% agarose gel.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Illumina Totalprep kit
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 3 of WTC
Data processing The data were normalised using quantile normalisation using Illumina's BeadStudio software
 
Submission date Feb 24, 2010
Last update date Feb 24, 2010
Contact name Yoonkwang Lee
E-mail(s) ylee3@neomed.edu
Organization name Northeast Ohio Medical University
Department Integrative Medical Sciences
Street address 4209 State Route 44
City Rootstown
ZIP/Postal code 44272
Country USA
 
Platform ID GPL6887
Series (1)
GSE20518 mRNA expression in islets isolated from wild type and SHPKO mice fed chow or western diet

Data table header descriptions
ID_REF
VALUE quantile-normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2735294 28.8 0.00962
ILMN_2417611 -3.2 0.6485
ILMN_2545897 58.3 0
ILMN_2762289 -2.5 0.59936
ILMN_1248788 -3.2 0.6485
ILMN_2707227 1.3 0.38462
ILMN_2896528 2191 0
ILMN_2721178 629.8 0
ILMN_1227723 275 0
ILMN_2458837 -2.2 0.57799
ILMN_3033922 1476.2 0
ILMN_3092673 6795.3 0
ILMN_1230777 4164.2 0
ILMN_2730714 4544.5 0
ILMN_1246069 1090.6 0
ILMN_1232042 -7.8 0.88355
ILMN_1243193 1629.8 0
ILMN_2524361 3.4 0.28526
ILMN_1233188 54.9 0
ILMN_2543688 405.7 0

Total number of rows: 45281

Table truncated, full table size 1067 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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