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Sample GSM515400 Query DataSets for GSM515400
Status Public on Feb 24, 2010
Title SHPKO_WD_rep2
Sample type RNA
 
Source name mouse islets
Organism Mus musculus
Characteristics gender: male
strain: C57Bl/6Hsd
age: 10 months
tissue: islets
genotype/variation: SHPKO
growth protocol: Western diet
Treatment protocol To isolate the islets from pancreas, pancreas were perfused with collagenase solution.
Growth protocol Mouse were fed chow and a western diet for 6 months. The diet regimen started when they were at 4 month old
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed using a 1% agarose gel.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Illumina Totalprep kit
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 2 of KOWD
Data processing The data were normalised using quantile normalisation using Illumina's BeadStudio software
 
Submission date Feb 24, 2010
Last update date Feb 24, 2010
Contact name Yoonkwang Lee
E-mail(s) ylee3@neomed.edu
Organization name Northeast Ohio Medical University
Department Integrative Medical Sciences
Street address 4209 State Route 44
City Rootstown
ZIP/Postal code 44272
Country USA
 
Platform ID GPL6887
Series (1)
GSE20518 mRNA expression in islets isolated from wild type and SHPKO mice fed chow or western diet

Data table header descriptions
ID_REF
VALUE quantile-normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2735294 0.9 0.39637
ILMN_2417611 6.2 0.18056
ILMN_2545897 46.4 0
ILMN_2762289 5.8 0.19017
ILMN_1248788 -4.5 0.72009
ILMN_2707227 2.7 0.30769
ILMN_2896528 2339.6 0
ILMN_2721178 482.9 0
ILMN_1227723 222.7 0
ILMN_2458837 -7.4 0.86966
ILMN_3033922 1222.2 0
ILMN_3092673 7006.1 0
ILMN_1230777 4237.9 0
ILMN_2730714 4118.6 0
ILMN_1246069 747.1 0
ILMN_1232042 -8.3 0.90278
ILMN_1243193 1438.3 0
ILMN_2524361 2 0.34188
ILMN_1233188 87.4 0
ILMN_2543688 647.4 0

Total number of rows: 45281

Table truncated, full table size 1062 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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