|
Status |
Public on Sep 16, 2011 |
Title |
N2 vs. mir-243_Replicate2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
N2
|
Organism |
Caenorhabditis elegans |
Characteristics |
strain: N2 genotype: wild-type developmental stage: young adult
|
Treatment protocol |
The mir-243 allele used in this study was n4759.
|
Growth protocol |
Nematodes were cultured according to standard procedures. The Bristol strain N2 was used as the standard wild-type strain.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA from nematodes were isolated using Trizol LS (Invitrogen) according to the manufacturer’s protocol.
|
Label |
Cy3
|
Label protocol |
500 ng of RNA samples from wild-type and mir-243 young-adult worms were reverse-transcribed with random primers. Each cDNA sample was split and labeled with both Cy3 and Cy5.
|
|
|
Channel 2 |
Source name |
mir-243 mutant
|
Organism |
Caenorhabditis elegans |
Characteristics |
genotype: mir-243 mutant developmental stage: young adult
|
Treatment protocol |
The mir-243 allele used in this study was n4759.
|
Growth protocol |
Nematodes were cultured according to standard procedures. The Bristol strain N2 was used as the standard wild-type strain.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA from nematodes were isolated using Trizol LS (Invitrogen) according to the manufacturer’s protocol.
|
Label |
Cy5
|
Label protocol |
500 ng of RNA samples from wild-type and mir-243 young-adult worms were reverse-transcribed with random primers. Each cDNA sample was split and labeled with both Cy3 and Cy5.
|
|
|
|
Hybridization protocol |
Samples were hybridized to Agilent C. elegans whole genome arrays at 65ºC overnight.
|
Scan protocol |
Scanned on an Agilent G2565AA scanner. Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
|
Description |
Biological replicate 2 of 3.
|
Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization. Txt files were then analyzed by the GeneSpring software.
|
|
|
Submission date |
Feb 28, 2010 |
Last update date |
Sep 16, 2011 |
Contact name |
Rene Ketting |
E-mail(s) |
r.ketting@hubrecht.eu
|
Organization name |
Hubrecht Institute
|
Street address |
Uppsalalaan 8
|
City |
Utrecht |
ZIP/Postal code |
3584CT |
Country |
Netherlands |
|
|
Platform ID |
GPL8209 |
Series (1) |
GSE20558 |
Adult Caenorhabditis elegans: wild-type (N2) and mir-243 mutant worms |
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