|
| Status |
Public on Sep 16, 2011 |
| Title |
N2 vs. mir-243_Replicate3_dye swap |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
N2
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: N2
genotype: wild-type
developmental stage: young adult
|
| Treatment protocol |
The mir-243 allele used in this study was n4759.
|
| Growth protocol |
Nematodes were cultured according to standard procedures. The Bristol strain N2 was used as the standard wild-type strain.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from nematodes were isolated using Trizol LS (Invitrogen) according to the manufacturer’s protocol.
|
| Label |
Cy5
|
| Label protocol |
500 ng of RNA samples from wild-type and mir-243 young-adult worms were reverse-transcribed with random primers. Each cDNA sample was split and labeled with both Cy3 and Cy5.
|
| |
|
| Channel 2 |
| Source name |
mir-243 mutant
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
genotype: mir-243 mutant
developmental stage: young adult
|
| Treatment protocol |
The mir-243 allele used in this study was n4759.
|
| Growth protocol |
Nematodes were cultured according to standard procedures. The Bristol strain N2 was used as the standard wild-type strain.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from nematodes were isolated using Trizol LS (Invitrogen) according to the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
500 ng of RNA samples from wild-type and mir-243 young-adult worms were reverse-transcribed with random primers. Each cDNA sample was split and labeled with both Cy3 and Cy5.
|
| |
|
| |
| Hybridization protocol |
Samples were hybridized to Agilent C. elegans whole genome arrays at 65ºC overnight.
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner. Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
|
| Description |
Biological replicate 3 of 3 - dye-swap.
|
| Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization. Txt files were then analyzed by the GeneSpring software.
|
| |
|
| Submission date |
Feb 28, 2010 |
| Last update date |
Sep 16, 2011 |
| Contact name |
Rene Ketting |
| E-mail(s) |
r.ketting@hubrecht.eu
|
| Organization name |
Hubrecht Institute
|
| Street address |
Uppsalalaan 8
|
| City |
Utrecht |
| ZIP/Postal code |
3584CT |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL8209 |
| Series (1) |
| GSE20558 |
Adult Caenorhabditis elegans: wild-type (N2) and mir-243 mutant worms |
|
