|
| Status |
Public on Mar 31, 2010 |
| Title |
Ferret lung with A/Brisbane/59/2007 (H1N1) on Day 5, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Ferret
|
| Organism |
Mustela putorius furo |
| Characteristics |
tissue: lung
virus strain: A/Brisbane/59/2007 (H1N1)
|
| Treatment protocol |
Intranasal infection of ferrets with 10^6 EID50 A/California/07/2009 (H1N1) for the first experiment and 10^6 EID50 A/Brisbane/59/2007 (H1N1) for the second experiment. On Day 0 (before infection), Day 3 (post infection for the first experiment) or Day 2 (post infection for the second experiment), and Day 5, Day 7 and Day 14 (post infection for both experiments), ferrets were euthanized and lung tissue was excised and put in RNAlater (Ambion, Austin, TX) immediately.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol extraction of total RNA from lung was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix Two-Cycle protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Canine Genome 2.0 Array in Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from ferret lung on Day 5 after infection
|
| Data processing |
The data were analyzed with PLIER using Expression Console Version 1.1 (Affymetrix) default analysis settings.
|
| |
|
| Submission date |
Mar 02, 2010 |
| Last update date |
Mar 31, 2010 |
| Contact name |
Longsi Ran |
| E-mail(s) |
lran@uhnresearch.ca
|
| Phone |
416-581-7607
|
| Organization name |
Toronto General Research Institute
|
| Department |
Division of Experimental Therapeutics
|
| Lab |
Dr. David J Kelvin
|
| Street address |
101 College Street
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL3738 |
| Series (1) |
| GSE17079 |
Early CCL2 and CXCL10 chemokine expression correlates with lung pathology in Pandemic H1N1 Influenza A infection |
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