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Sample GSM517159 Query DataSets for GSM517159
Status Public on Mar 31, 2010
Title Ferret lung with A/Brisbane/59/2007 (H1N1) on Day 14, biological rep2
Sample type RNA
 
Source name Ferret
Organism Mustela putorius furo
Characteristics tissue: lung
virus strain: A/Brisbane/59/2007 (H1N1)
Treatment protocol Intranasal infection of ferrets with 10^6 EID50 A/California/07/2009 (H1N1) for the first experiment and 10^6 EID50 A/Brisbane/59/2007 (H1N1) for the second experiment. On Day 0 (before infection), Day 3 (post infection for the first experiment) or Day 2 (post infection for the second experiment), and Day 5, Day 7 and Day 14 (post infection for both experiments), ferrets were euthanized and lung tissue was excised and put in RNAlater (Ambion, Austin, TX) immediately.
Extracted molecule total RNA
Extraction protocol TRIzol extraction of total RNA from lung was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix Two-Cycle protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Canine Genome 2.0 Array in Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Description Gene expression data from ferret lung on Day 14 after infection
Data processing The data were analyzed with PLIER using Expression Console Version 1.1 (Affymetrix) default analysis settings.
 
Submission date Mar 02, 2010
Last update date Mar 31, 2010
Contact name Longsi Ran
E-mail(s) lran@uhnresearch.ca
Phone 416-581-7607
Organization name Toronto General Research Institute
Department Division of Experimental Therapeutics
Lab Dr. David J Kelvin
Street address 101 College Street
City Toronto
State/province ON
ZIP/Postal code M5G 1L7
Country Canada
 
Platform ID GPL3738
Series (1)
GSE17079 Early CCL2 and CXCL10 chemokine expression correlates with lung pathology in Pandemic H1N1 Influenza A infection

Data table header descriptions
ID_REF
VALUE PLIER-calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 119.7913
AFFX-BioB-5_at 127.0005
AFFX-BioB-M_at 160.9169
AFFX-BioC-3_at 347.9058
AFFX-BioC-5_at 290.1763
AFFX-BioDn-3_at 962.6909
AFFX-BioDn-5_at 744.9366
AFFX-Cfa-actin-3_s_at 1703.938
AFFX-Cfa-actin-5_at 484.0663
AFFX-Cfa-actin-5_s_at 0.331838
AFFX-Cfa-actin-M_x_at 1269.256
AFFX-Cfa-ef1a-3_x_at 4242.778
AFFX-Cfa-ef1a-5_at 3.872418
AFFX-Cfa-ef1a-M_x_at 1724.46
AFFX-Cfa-gapdh-3_x_at 143.3945
AFFX-Cfa-gapdh-5_x_at 4.925736
AFFX-Cfa-gapdh-M_x_at 22.23668
AFFX-Cfa-r2-Bs-dap-3_at 5.022322
AFFX-Cfa-r2-Bs-dap-5_at 8.902533
AFFX-Cfa-r2-Bs-dap-M_at 2.621886

Total number of rows: 43035

Table truncated, full table size 1232 Kbytes.




Supplementary file Size Download File type/resource
GSM517159.CEL.gz 3.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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