|
| Status |
Public on Mar 03, 2012 |
| Title |
miR-122KO liver RNA4 |
| Sample type |
RNA |
| |
|
| Source name |
8 week old male
|
| Organism |
Mus musculus |
| Characteristics |
genotype: mIR-122 KO
genetic background: 57/BL6J
tissue: liver
|
| Treatment protocol |
None
|
| Growth protocol |
8 week old male mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated from livers using miRVana kit from Applied Biosystems
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Herring sperm DNA, Acetylated BSA (50mg/mL), BioArray Eukaryotic Hybridization Controls (Enzo Life Sciences), heated at 65oC for 5 minutes, 99oC for 5 minutes, 45oC for 5 minutes centrifuged at maximum speed for 5 minutes, hybridized for 16 hours at 45° at 60rpm. Fluidics washing is FS450_0001
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G using GCOS version 1.4.0.036
|
| Description |
n/a
|
| Data processing |
Data were processed using Affymetrix Expression Console software. Robust Multichip Average (RMA) method was used to do background correction, quantile normalization, and expression level summarization
|
| |
|
| Submission date |
Mar 03, 2010 |
| Last update date |
Mar 03, 2012 |
| Contact name |
kalpana ghoshal |
| E-mail(s) |
kalpana.ghoshal@osumc.edu
|
| Phone |
614-292-8865
|
| Fax |
614-688-5600
|
| Organization name |
The Ohio State University
|
| Department |
Molecular and Cellular Biochemistry
|
| Street address |
420 W. 12th Avenue
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE20610 |
Liver transcriptome profiling of liver specific miR-122 knockout and control male mice |
|
