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Sample GSM518352 Query DataSets for GSM518352
Status Public on Apr 01, 2010
Title Wild Type, 2
Sample type RNA
 
Source name quadriceps femoris
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: Wild Type
replicate: 2
Growth protocol Mice (backcrossed C57Bl/6J background) were housed in QBP vivarium (University of Queensland, St. Lucia, Queensland, Australia) with 12h light–dark cycle and fed a standard diet containing 4.6% total fat (from Specialty Feeds, Glen Forrest, Western Australia) and water provided ad libitum.
Extracted molecule total RNA
Extraction protocol Mice were overnight fasted and then sacrificed at 9:00AM in the morning. RNA was extracted from snap frozen quadriceps femoris with tri-reagent (Sigma), followed by treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser 2100.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification kit (Ambion)
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Scanned with Illumina BeadStation Scanner.
Description 4068846001_B
Data processing Data was compiled using BeadStudio v2.3.41 (Illumina). Normalized data was produced using GeneSpring GX version 11 via normalization to control genes, where control genes were represented by all genes with an Illumina detection score equal to one in at least three out of the six samples (7730 control genes in total).
 
Submission date Mar 05, 2010
Last update date Mar 05, 2010
Contact name Michael Andrew Pearen
E-mail(s) m.pearen@uq.edu.au
Phone +61 7 33462365
Fax +61 7 33462101
Organization name Institute for Molecular Bioscience
Lab Group Muscat
Street address The University of Queensland
City St Lucia
State/province Queensland
ZIP/Postal code 4072
Country Australia
 
Platform ID GPL6105
Series (1)
GSE20646 Identification and validation of the pathways and functions regulated by the ROR alpha1 in skeletal muscle

Data table header descriptions
ID_REF
VALUE Normalized data

Data table
ID_REF VALUE
ILMN_1219891 -0.21883512
ILMN_2504945 -0.071181774
ILMN_2481381 -0.006638765
ILMN_2514482 -0.109084606
ILMN_2508277 0.04021454
ILMN_2516718 0.038835764
ILMN_2423589 -0.13240552
ILMN_2518810 -0.04270029
ILMN_2520883 0.0590384
ILMN_1253780 0.004064322
ILMN_2487542 -0.047516584
ILMN_2446717 0.020923615
ILMN_2503259 -0.020792246
ILMN_1242708 -0.12775731
ILMN_2478051 -0.001616716
ILMN_2426740 -0.08604312
ILMN_2478074 -0.22118783
ILMN_2440107 -0.07879543
ILMN_1230073 0.030794144
ILMN_2638923 -0.41099262

Total number of rows: 46632

Table truncated, full table size 1132 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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