|
| Status |
Public on Apr 14, 2010 |
| Title |
N2 |
| Sample type |
SRA |
| |
|
| Source name |
small RNA from N2 wild-type adults
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: N2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol reagent and small RNA from 18-30 nucleotides were purified from gel. For cDNA synthesis, the RNA molecules in this fraction were first poly A-tailed using poly(A)polymerase followed by ligation of synthetic RNA adapter to the 5' phosphate of the miRNAs. First strand cDNA synthesis was then performed using an oligo(dT)-linker primer and M-MLVRNase H- reverse transcriptase. cDNA was PCR-amplified with adapter-specific primers and used in Illumina sequencing.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
Small RNA sequence data
|
| Data processing |
3' adapter sequences were trimmed and inserts longer than 18 nt were mapped to the C. elegans genome (ce4).
|
| |
|
| Submission date |
Mar 05, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Eugene Berezikov |
| E-mail(s) |
e.berezikov@umcg.nl
|
| Phone |
+31 50 361 73 00
|
| Organization name |
University Medical Center Groningen
|
| Department |
European Research Institute for the Biology of Ageing
|
| Street address |
Antonius Deusinglaan 1
|
| City |
Groningen |
| ZIP/Postal code |
9713AV |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL9269 |
| Series (1) |
| GSE20649 |
MicroRNA-Directed siRNA Biogenesis in Caenorhabditis elegans |
|
| Relations |
| SRA |
SRX019243 |
| BioSample |
SAMN00011625 |
