|
Status |
Public on Sep 15, 2010 |
Title |
Gonadal fat (SAMPLE 4) |
Sample type |
RNA |
|
|
Source name |
Mouse gonadal fat
|
Organism |
Mus musculus |
Characteristics |
gender: female strain: M16i x MB2 F2 intercross tissue: Gonadal fat haplotype class: 4 scanning day: 3 scanning position: 4 age: 15 weeks
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with Trizol reagent, following manufacturer´s protocol. Quality control was performed with Agilent 2100 Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinilated cRNA syntheses were generated using the Ambion Total Prep RNA Amplification Kit for Illumina arrays following the manufacturer´s instructions.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
10210
|
Data processing |
The data were normalized using the Loess followed by the Robust Spline Normalization (RSN) methods with the Lumi package in R.
|
|
|
Submission date |
Mar 10, 2010 |
Last update date |
Mar 10, 2010 |
Contact name |
Gloria Muñoz |
E-mail(s) |
munoz.gloria@inia.es
|
Phone |
+34-913473792
|
Fax |
+34-913474192
|
Organization name |
INIA
|
Department |
Mejora Genetica Animal
|
Street address |
Crta. Coruña km 7
|
City |
Madrid |
ZIP/Postal code |
28041 |
Country |
Spain |
|
|
Platform ID |
GPL4234 |
Series (1) |
GSE20724 |
Genome-wide expression analysis of mouse chromosome 2 in gonadal fat and liver tissues reveals a complex architecture of obesity |
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