|
| Status |
Public on Sep 15, 2010 |
| Title |
Gonadal fat (SAMPLE 5) |
| Sample type |
RNA |
| |
|
| Source name |
Mouse gonadal fat
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: M16i x MB2 F2 intercross
tissue: Gonadal fat
haplotype class: 1
scanning day: 3
scanning position: 5
age: 15 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol reagent, following manufacturer´s protocol. Quality control was performed with Agilent 2100 Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinilated cRNA syntheses were generated using the Ambion Total Prep RNA Amplification Kit for Illumina arrays following the manufacturer´s instructions.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
12403
|
| Data processing |
The data were normalized using the Loess followed by the Robust Spline Normalization (RSN) methods with the Lumi package in R.
|
| |
|
| Submission date |
Mar 10, 2010 |
| Last update date |
Mar 10, 2010 |
| Contact name |
Gloria Muñoz |
| E-mail(s) |
munoz.gloria@inia.es
|
| Phone |
+34-913473792
|
| Fax |
+34-913474192
|
| Organization name |
INIA
|
| Department |
Mejora Genetica Animal
|
| Street address |
Crta. Coruña km 7
|
| City |
Madrid |
| ZIP/Postal code |
28041 |
| Country |
Spain |
| |
|
| Platform ID |
GPL4234 |
| Series (1) |
| GSE20724 |
Genome-wide expression analysis of mouse chromosome 2 in gonadal fat and liver tissues reveals a complex architecture of obesity |
|
