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Sample GSM520324 Query DataSets for GSM520324
Status Public on Sep 15, 2010
Title Gonadal fat (SAMPLE 40)
Sample type RNA
 
Source name Mouse gonadal fat
Organism Mus musculus
Characteristics gender: male
strain: M16i x MB2 F2 intercross
tissue: Gonadal fat
haplotype class: 1
scanning day: 8
scanning position: 4
age: 15 weeks
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Trizol reagent, following manufacturer´s protocol. Quality control was performed with Agilent 2100 Bioanalyser.
Label biotin
Label protocol Biotinilated cRNA syntheses were generated using the Ambion Total Prep RNA Amplification Kit for Illumina arrays following the manufacturer´s instructions.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description 43004
Data processing The data were normalized using the Loess followed by the Robust Spline Normalization (RSN) methods with the Lumi package in R.
 
Submission date Mar 10, 2010
Last update date Mar 10, 2010
Contact name Gloria Muñoz
E-mail(s) munoz.gloria@inia.es
Phone +34-913473792
Fax +34-913474192
Organization name INIA
Department Mejora Genetica Animal
Street address Crta. Coruña km 7
City Madrid
ZIP/Postal code 28041
Country Spain
 
Platform ID GPL4234
Series (1)
GSE20724 Genome-wide expression analysis of mouse chromosome 2 in gonadal fat and liver tissues reveals a complex architecture of obesity

Data table header descriptions
ID_REF
VALUE Loess/RSN normalized signal intensity

Data table
ID_REF VALUE
102450270 7.245782416
104200035 7.27992854
100730138 7.246150038
102320427 7.173666337
102630735 7.018906793
100450435 7.265794256
101230594 7.054423308
100780541 7.092454222
104200184 7.098726267
105360286 7.084736186
103060411 7.261255354
100630193 7.035524891
105270286 7.124859967
101690685 7.056967732
100060180 7.273084444
107100563 7.127231105
100450451 7.144935976
100610092 6.956817243
104810452 11.25703569
6840019 9.548473187

Total number of rows: 46116

Table truncated, full table size 935 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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