|
| Status |
Public on Apr 15, 2010 |
| Title |
Untreated-rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Untreated
|
| Organism |
Mus musculus |
| Characteristics |
source tissue: Forelimb and hindlimb buds
cell type: Primary limb bud cells
developmental stage: embyonic day 11.5
strain: 129XB6-Sox4fl/fl11fl/fl
genotype: Wild type
|
| Treatment protocol |
Primary limb bud cells were left untreated, or were treated with LacZ or Cre expressing adenovirus for 24h
|
| Growth protocol |
Primary limb bud cells were isolated from E11.5 mouse embryos and cultured for 6h before treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
cRNA labeled with Biotin was synthesized according to direct hybridization assay protocol from Illumina
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate-2
|
| Data processing |
The data were normalised using quantile normalisation with Illumina Beadstudio 2.1
|
| |
|
| Submission date |
Mar 10, 2010 |
| Last update date |
Mar 31, 2010 |
| Contact name |
Pallavi Bhattaram |
| E-mail(s) |
bhattap2@ccf.org
|
| Organization name |
Cleveland Clinic
|
| Street address |
9500 Euclid Avenue
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44120 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE20740 |
Organogenesis relies on the SoxC transcription factors for the survival of neural and mesenchymal progenitors |
|
