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Sample GSM5211997 Query DataSets for GSM5211997
Status Public on Jan 11, 2022
Title WT_TolLPS_1_1
Sample type SRA
 
Source name BMDC
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: BMDC
genotype: wild type
treatment: LPS + IL10 + TGF-β
Treatment protocol Five biological replicates with treatments for 8 hours.
Growth protocol Bone marrow derive dendritic cells were routinely generated by re-plating d9 bone marrow cell differentiation cultures (typically 2–3 × 106 cells into 100mm diameter dish) on uncoated plastic in complete RPMI1640 (Gibco) supplemented with 20 ng/ml of GM-CSF (PeproTech).
Extracted molecule total RNA
Extraction protocol BMDCs were harvest, live CD11c hi BMDCs were sorted into lysis buffer, flash frozen on dry ice. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina Genome Analyzer II
 
Data processing Bcl2Fastq
RSEM Gene quantification V1.2.8
Genome_build: mm10
 
Submission date Mar 25, 2021
Last update date Jan 11, 2022
Contact name Ayshwarya Subramanian
E-mail(s) subraman@broadinstitute.org
Organization name Broad Institute
Department Klarman Cell Observatory
Street address 415 Main Street
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL9250
Series (2)
GSE169663 Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Bat3-/- BMDC Transcriptomes III
GSE169664 Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Bat3-/- BMDC Transcriptomes
Relations
BioSample SAMN18492538
SRA SRX10447027

Supplementary file Size Download File type/resource
GSM5211997_Bulk_Bat3_Tim3_In_BMDC_WT_TolLPS_1_1_RSEM_mm10_ucsc_genomestudio_genes_RSEM_mm10.genes.results.txt.gz 427.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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