|
| Status |
Public on Mar 22, 2010 |
| Title |
macrophages |
| Sample type |
RNA |
| |
|
| Source name |
macrophages cultured in the presence of M-CSF for six days
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Bone marrow derived macrophages
strain: C57/Black6
|
| Treatment protocol |
Common progenitor cells were cultured with M-CSF alone for macrophages and M-CSF plus RANKL for osteoclasts, respectively. After six days of cultivation, cells were lysed in RLT solution (RNeasy, QIAGEN).
|
| Growth protocol |
Bone marrow cells were isolated from 8 weeks old mouse and cultured in the presence of M-CSF for three days. Then M-CSF-dependent adherent cells were harvested as macrophage and osteoclast common progenitor cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RLT extraction of total RNA was performed according to the manufacturer's instructions (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol, one-cycle, from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip®Mouse Genome 430 2.0 Array. GeneChip® Arrays were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips® Arrays were scanned using the GeneChip® Scanner 3000 7G.
|
| Description |
Gene expression data from macrophages
|
| Data processing |
The data were analyzed with GeneChip®Operating Software using Affymetrix default analysis settings.
|
| |
|
| Submission date |
Mar 12, 2010 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Takeshi Miyamoto |
| E-mail(s) |
miyamoto@sc.itc.keio.ac.jp
|
| Phone |
81-3-3353-1252
|
| Fax |
81-3-3353-6597
|
| Organization name |
Keio University
|
| Department |
School of Medicine
|
| Lab |
Department of Orthopedic Surgery
|
| Street address |
35 Shinanomachi
|
| City |
Shinjuku-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
160－8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE20850 |
Expression data from cultured macrophages and osteoclasts |
|
| Relations |
| Reanalyzed by |
GSE119085 |
| Reanalyzed by |
GSE135324 |
