|
| Status |
Public on Nov 10, 2010 |
| Title |
Liver_LEP_SQ_1029 |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: ob/ob
treatment: Leptin
route: SQ
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRI REAGENT (Sigma), according to the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
Purified RNA (200 ng) was further used for cRNA amplification and labeling with biotin using Target Amp cDNA synthesis kit (Epicenter catalog no. TAB1R6924).
|
| |
|
| Hybridization protocol |
Approximately 750ng of labeled cRNA was hybridized to the MouseRef-8 v2.0 Expression BeadChips as per manufacturer’s instructions.
|
| Scan protocol |
After hybridization, arrays were washed, stained with Cy3-conjugated streptavidin, and scanned.
|
| Data processing |
Image data were analyzed with BeadStudio V2.0. Raw intensity data from BeadStudio were exported for further statistical analyses. Microarray data were normalized using the lumi package in R, using the variance stabilizing transformation (VST) of the package and robust spline normalization (RSN).
|
| |
|
| Submission date |
Mar 15, 2010 |
| Last update date |
Mar 15, 2010 |
| Contact name |
Jin-Xiong She |
| E-mail(s) |
jshe@gru.edu
|
| Phone |
706-721-3410
|
| Fax |
706-721-3688
|
| URL |
http://www.cbgm.mcg.edu/
|
| Organization name |
Medical College of Georgia
|
| Department |
Center for Biotechnology and Genomic Medicine
|
| Street address |
1120 15th Street, CA4124
|
| City |
Augusta |
| State/province |
GA |
| ZIP/Postal code |
30912-2400 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE20878 |
Hepatic gene expression profiling reveals key pathways involved in leptin mediated weight loss in ob/ob mice. |
|
