|
| Status |
Public on Mar 15, 2010 |
| Title |
Control_ES-LIF(+)_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Naïve murine stem cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: ES-LIF(+)
|
| Growth protocol |
Cell culture derived from inner cell mass of murine blastocysts.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA using the Invitrogen Micro-to-Midi kit, performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Murine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from naïve stem cells
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Mar 15, 2010 |
| Last update date |
Mar 22, 2010 |
| Contact name |
Atta Behfar |
| E-mail(s) |
behfar.atta@mayo.edu
|
| Organization name |
Mayo Clinic
|
| Street address |
200 First Street SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6689 |
Expression data during stem cell differentiation |
|
