|
| Status |
Public on Mar 16, 2010 |
| Title |
mPFC DT4+1 5 |
| Sample type |
RNA |
| |
|
| Source name |
medial prefrontal cortex, 1-day abstinence
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
tissue: medial prefrontal cortex
experimental group: cocaine abstinence 1 day
gender: male
|
| Treatment protocol |
No cocaine, or 10 days of cocaine self-administration followed by 1, 10 or 100 days of enforced abstinence.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tri-Reagent/BCP (Molecular Research Center, Cincinnati, OH). Cleanup with Qiagen Rneasy final step (Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA was prepared by linear amplification of the Poly(A)+ RNA population within the total RNA sample.
|
| |
|
| Hybridization protocol |
Arrays were hybridized at 37C for 18h in a shaking incubator, and stained with streptavidin-Alexa 647.
|
| Scan protocol |
Arrays were scanned with Axon GenePixPro 4000B (Molecular Devices, Axon Instruments, Union City, CA). Images were processed using CodeLink Expression Analysis Software.
|
| Description |
Replicate 5 of 6.
|
| Data processing |
Normalized values represent median global normalization method, i.e., background-subtracted raw intensity/median intensity of all spots on the array. Genespring Gx 7.3 was used for normalization.
|
| |
|
| Submission date |
Mar 15, 2010 |
| Last update date |
Mar 15, 2010 |
| Contact name |
Penn State COM |
| Organization name |
Penn State College of Medicine
|
| Department |
Genome Sciences Facility
|
| Street address |
500 University Drive
|
| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
| |
|
| Platform ID |
GPL2896 |
| Series (1) |
| GSE20885 |
Gene expression changes in the medial prefrontal cortex and nucleus accumbens following abstinence from cocaine self-administration |
|
