strain: Sprague-Dawley tissue: nucleus accumbens experimental group: Naïve gender: male
Treatment protocol
No cocaine, or 10 days of cocaine self-administration followed by 1, 10 or 100 days of enforced abstinence.
Extracted molecule
total RNA
Extraction protocol
Tri-Reagent/BCP (Molecular Research Center, Cincinnati, OH). Cleanup with Qiagen Rneasy final step (Valencia, CA).
Label
biotin
Label protocol
Biotin-labeled cRNA was prepared by linear amplification of the Poly(A)+ RNA population within the total RNA sample.
Hybridization protocol
Arrays were hybridized at 37C for 18h in a shaking incubator, and stained with streptavidin-Alexa 647.
Scan protocol
Arrays were scanned with Axon GenePixPro 4000B (Molecular Devices, Axon Instruments, Union City, CA). Images were processed using CodeLink Expression Analysis Software.
Description
Replicate 3 of 5.
Data processing
Normalized values represent median global normalization method, i.e., background-subtracted raw intensity/median intensity of all spots on the array. Genespring Gx 7.3 was used for normalization.
