|
| Status |
Public on Mar 17, 2010 |
| Title |
2wk post 3 |
| Sample type |
RNA |
| |
|
| Source name |
thoracic transected spinal cord, 2 week post lesion
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell type: laser-dissected neurons from spinal cord
strain: Long Evans
gender: Female
age: 77 +/- 10 days
injury: thoracic transected spinal cord
time: 2 week post lesion
|
| Treatment protocol |
Thoracic proporiospinal neurons that project to the lumbar enlargement were retrogradely labeled by fluorescent tracer injection prior to transection of the spinal cord and subsequently laser-dissected following different post-lesion or control survival times
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Nugen WT Ovation Pico Kit protocol with Exon Module Kit, from 50 nanograms total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.75 micrograms of cDNA were hybridized for 16 hr at 45C on Rat ST Gene Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 7G/4C scanner.
|
| Description |
propriospinal neuron gene expression data at different time points following spinal cord transection or sham. Additional details in published paper.
|
| Data processing |
The data were imported into GeneSpring GX (Agilent) and RMA normalized.
|
| |
|
| Submission date |
Mar 16, 2010 |
| Last update date |
Mar 16, 2010 |
| Contact name |
Frank A Middleton |
| E-mail(s) |
middletf@upstate.edu
|
| Phone |
315-464-7721
|
| Organization name |
SUNY Upstate Medical Univ
|
| Department |
Neuroscience and Physiology
|
| Street address |
505 Irving Avenue
|
| City |
Syracuse |
| State/province |
NY |
| ZIP/Postal code |
13210 |
| Country |
USA |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE20907 |
Intrinsic response of thoracic propriospinal neurons to axotomy |
|
