|
| Status |
Public on Jul 01, 2010 |
| Title |
Stomach_Car9ko_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse stomach, Car9 knockout
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: C57BL/6
genotype: Car9-/-
tissue: stomach
age: 10 months
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Rneasy RNA isolation kit including Rnase-free Dnase treatment (Qiagen) according to the manufacturer's protocol. Concentration and purity were measured with Nanodrop.
|
| Label |
biotin
|
| Label protocol |
Using Illumina TotalPrep RNA Amplification kit (#AMIL1791, Ambion), following the manufacturer's protocol. Input amount: 400ng of total RNA. Incubation of labeling reaction: 14.5h.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol. 18h at 58 degrees.
|
| Scan protocol |
Standard scan factor in Illumina BeadArray reader.
|
| Description |
Replicate 1 of 6.
|
| Data processing |
Array data were normalized with Chipster version 1.3.0 using the quantile normalization method.
|
| |
|
| Submission date |
Mar 19, 2010 |
| Last update date |
May 31, 2010 |
| Contact name |
Heini Kallio |
| E-mail(s) |
heini.kallio@uta.fi
|
| Organization name |
University of Tampere
|
| Department |
Institute of Medical Technology and School of Medicine
|
| Street address |
Biokatu 6
|
| City |
Tampere |
| ZIP/Postal code |
33520 |
| Country |
Finland |
| |
|
| Platform ID |
GPL6481 |
| Series (1) |
| GSE20981 |
Global transcriptional response to carbonic anhydrase IX deficiency in the mouse stomach |
|
