|
Status |
Public on Jul 01, 2010 |
Title |
Stomach_Car9ko_rep4 |
Sample type |
RNA |
|
|
Source name |
Mouse stomach, Car9 knockout
|
Organism |
Mus musculus |
Characteristics |
gender: male strain: C57BL/6 genotype: Car9-/- tissue: stomach age: 12 months
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Rneasy RNA isolation kit including Rnase-free Dnase treatment (Qiagen) according to the manufacturer's protocol. Concentration and purity were measured with Nanodrop.
|
Label |
biotin
|
Label protocol |
Using Illumina TotalPrep RNA Amplification kit (#AMIL1791, Ambion), following the manufacturer's protocol. Input amount: 400ng of total RNA. Incubation of labeling reaction: 14.5h.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol. 18h at 58 degrees.
|
Scan protocol |
Standard scan factor in Illumina BeadArray reader.
|
Description |
Replicate 4 of 6.
|
Data processing |
Array data were normalized with Chipster version 1.3.0 using the quantile normalization method.
|
|
|
Submission date |
Mar 19, 2010 |
Last update date |
May 31, 2010 |
Contact name |
Heini Kallio |
E-mail(s) |
heini.kallio@uta.fi
|
Organization name |
University of Tampere
|
Department |
Institute of Medical Technology and School of Medicine
|
Street address |
Biokatu 6
|
City |
Tampere |
ZIP/Postal code |
33520 |
Country |
Finland |
|
|
Platform ID |
GPL6481 |
Series (1) |
GSE20981 |
Global transcriptional response to carbonic anhydrase IX deficiency in the mouse stomach |
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