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Sample GSM524616 Query DataSets for GSM524616
Status Public on Jul 01, 2010
Title Stomach_Car9ko_rep4
Sample type RNA
 
Source name Mouse stomach, Car9 knockout
Organism Mus musculus
Characteristics gender: male
strain: C57BL/6
genotype: Car9-/-
tissue: stomach
age: 12 months
Extracted molecule total RNA
Extraction protocol RNA was extracted with Rneasy RNA isolation kit including Rnase-free Dnase treatment (Qiagen) according to the manufacturer's protocol. Concentration and purity were measured with Nanodrop.
Label biotin
Label protocol Using Illumina TotalPrep RNA Amplification kit (#AMIL1791, Ambion), following the manufacturer's protocol. Input amount: 400ng of total RNA. Incubation of labeling reaction: 14.5h.
 
Hybridization protocol Standard Illumina hybridization protocol. 18h at 58 degrees.
Scan protocol Standard scan factor in Illumina BeadArray reader.
Description Replicate 4 of 6.
Data processing Array data were normalized with Chipster version 1.3.0 using the quantile normalization method.
 
Submission date Mar 19, 2010
Last update date May 31, 2010
Contact name Heini Kallio
E-mail(s) heini.kallio@uta.fi
Organization name University of Tampere
Department Institute of Medical Technology and School of Medicine
Street address Biokatu 6
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL6481
Series (1)
GSE20981 Global transcriptional response to carbonic anhydrase IX deficiency in the mouse stomach

Data table header descriptions
ID_REF
VALUE Quantile-normalized value
Detection Pval

Data table
ID_REF VALUE Detection Pval
106940692 6.17 0.5192433
580022 6.35 0.1728637
2940601 9.52 0
103440070 6.2 0.4481409
102260551 12.12 0
102370333 11.24 0
105670398 12.87 0
102030278 6.56 0.006523157
104670301 10.58 0
106770632 6.28 0.2778865
101770605 6.18 0.4964123
102320338 11.07 0
102470519 8 0
101940070 7.44 0
103710100 6.23 0.3907371
105720600 7.32 0
106840121 6.86 0
1240685 10.47 0
6450273 10.23 0
6020750 9.4 0

Total number of rows: 46643

Table truncated, full table size 935 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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