|
| Status |
Public on Mar 21, 2010 |
| Title |
Prkar1a+/- caudal vertebral bone lesion, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
vertebral bone, Prkar1a+/-
|
| Organism |
Mus musculus |
| Characteristics |
background: C57BL/6x129Sv/B6 hybrid
genotype: Prkar1a+/-
age: 9 months
tissue: caudal vertebral bone lesion
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
Replicate 1 of 3.
|
| Data processing |
Z score transformation for normalization was performed for each Illumina sample/array as previously described (Cheadle et al. J Mol Diagn, 5:73-81).
|
| |
|
| Submission date |
Mar 20, 2010 |
| Last update date |
Mar 20, 2010 |
| Contact name |
Kitman Tsang |
| E-mail(s) |
tsangkit@mail.nih.gov
|
| Phone |
301-451-8250
|
| Fax |
301-402-0574
|
| Organization name |
SEGEN/PDEGEN/NICHD/NIH
|
| Street address |
10 Center Drive, 10-CRC, Rm1E-3216
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE20984 |
Alternate protein kinase A activity identifies a unique population of stromal cells in adult bone |
|
