|
| Status |
Public on Sep 30, 2010 |
| Title |
Ko 2 |
| Sample type |
RNA |
| |
|
| Source name |
Peritoneal macrophages
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: peritoneal macrophages
genome/variation: CD13 knock out
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate Ko
|
| Data processing |
The data were normalised using quantile normalization using GenomeStudio.
|
| |
|
| Submission date |
Mar 20, 2010 |
| Last update date |
Mar 22, 2010 |
| Contact name |
Anupinder Kaur |
| E-mail(s) |
akaur@uchc.edu
|
| Phone |
(860)-679-8706
|
| Organization name |
UCHC
|
| Department |
Genetics and Developmental Biology
|
| Lab |
Translational Genomics Core
|
| Street address |
400 Farmington Avenue
|
| City |
Farmington |
| State/province |
CT |
| ZIP/Postal code |
06030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE20985 |
Comparison of gene expression in CD13 wild type versus Knock out Macrophages |
|
