|
| Status |
Public on Apr 19, 2021 |
| Title |
Liver_ApoE-KO_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
111018_osawa_05_ap3
|
| Organism |
Mus musculus |
| Characteristics |
genotype: ApoE-KO mouse liver with HFD
strain: C57BL/6J
age: adult
|
| Treatment protocol |
For liver samples, mice were feeded with HFD or 12 weeks.
|
| Growth protocol |
Maintain mice in normal circumstance (temperature and humidity). For aortic endothelial cell, primary endothalial cells were isolated from aorta of each mice, and cultured for several days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For liver sample, total RNA was extracted using RNeasy (QIAGEN) according to the manufacturer's instructions. For aorta sample, Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430.2.0 Array.
|
| Scan protocol |
GeneChips Scanner 3000 (Affymetrix)
|
| Data processing |
The data were analyzed with GeneSpringGX (Version12.5) using Affymetrix default analysis settings and global scaling as normalization method, and Transcriptome Analysis Console (TAC) software. For global normalization, the average signal in the array was set to 100
|
| |
|
| Submission date |
Apr 18, 2021 |
| Last update date |
Apr 19, 2021 |
| Contact name |
Takashi Minami |
| E-mail(s) |
t-minami@kumamoto-u.ac.jp
|
| Organization name |
Kumamoto University
|
| Street address |
2-2-1, Honjo
|
| City |
Kumamoto |
| ZIP/Postal code |
860-0811 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE172283 |
Loss of Down syndrome critical region-1 leads to cholesterol metabolic dysfunction that exaggerates hypercholesterolemia in ApoE-null background |
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