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Sample GSM5257935 Query DataSets for GSM5257935
Status Public on Apr 22, 2021
Title Cell_wildtype_day5
Sample type RNA
 
Source name preadipocytes_WT
Organism Mus musculus
Characteristics cell type: preadipocytes
genotype: wildtype
timepoint: Day 5
Treatment protocol No treatment
Growth protocol Preadipocytes were cultured with DMEM (high glucose) medium containing 10% NCS plus pen/strep, L-glutamine, 24 nM insulin, and 150 um sodium ascorbate. Medium was changed every two days.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from adipocytes at various days during differentiation with TRIzol reagent (Invitrogen)
Label Biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol
Description WT_D5
Data processing Raw data was background subtracted and normalized based on Cross-Correlation method.
The negative and small expression values were set to be 20 based on p value
 
Submission date Apr 21, 2021
Last update date Apr 22, 2021
Contact name Henry Yang
E-mail(s) csiyangh@nus.edu.sg
Organization name Cancer Science Institute of Singapore
Street address 28 Medical Drive
City Singapore
ZIP/Postal code 117456
Country Singapore
 
Platform ID GPL6885
Series (1)
GSE172491 Regulation of adipocyte differentiation by IRF3

Data table header descriptions
ID_REF
VALUE normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212607 20 0.32707
ILMN_1212612 73.266837 0.00752
ILMN_1212619 27.662287 0.05138
ILMN_1212628 20 0.698
ILMN_1212632 20 0.17293
ILMN_1212636 2943.7186 0
ILMN_1212637 2208.8949 0
ILMN_1212645 20 0.21303
ILMN_1212648 428.19168 0
ILMN_1212653 397.21757 0
ILMN_1212672 147.04634 0
ILMN_1212682 140.37846 0
ILMN_1212683 20 0.6817
ILMN_1212685 20 0.7995
ILMN_1212692 20 0.12657
ILMN_1212693 436.87917 0
ILMN_1212695 20 0.46115
ILMN_1212698 20 0.53383
ILMN_1212702 20 0.82957
ILMN_1212703 115.78823 0

Total number of rows: 25697

Table truncated, full table size 631 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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