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Sample GSM5257936 Query DataSets for GSM5257936
Status Public on Apr 22, 2021
Title Cell_wildtype_day7
Sample type RNA
Source name preadipocytes_WT
Organism Mus musculus
Characteristics cell type: preadipocytes
genotype: wildtype
timepoint: Day 7
Treatment protocol No treatment
Growth protocol Preadipocytes were cultured with DMEM (high glucose) medium containing 10% NCS plus pen/strep, L-glutamine, 24 nM insulin, and 150 um sodium ascorbate. Medium was changed every two days.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from adipocytes at various days during differentiation with TRIzol reagent (Invitrogen)
Label Biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol
Description WT_D7
Data processing Raw data was background subtracted and normalized based on Cross-Correlation method.
The negative and small expression values were set to be 20 based on p value
Submission date Apr 21, 2021
Last update date Apr 22, 2021
Contact name Henry Yang
Organization name Cancer Science Institute of Singapore
Street address 28 Medical Drive
City Singapore
ZIP/Postal code 117456
Country Singapore
Platform ID GPL6885
Series (1)
GSE172491 Regulation of adipocyte differentiation by IRF3

Data table header descriptions
VALUE normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212607 20 0.38596
ILMN_1212612 129.2187 0
ILMN_1212619 27.091136 0.03509
ILMN_1212628 20 0.8985
ILMN_1212632 20 0.13033
ILMN_1212636 3750.6436 0
ILMN_1212637 1761.5544 0
ILMN_1212645 21.070937 0.05263
ILMN_1212648 391.25663 0
ILMN_1212653 370.77847 0
ILMN_1212672 180.65246 0
ILMN_1212682 102.28617 0
ILMN_1212683 20 0.60025
ILMN_1212685 20 0.56892
ILMN_1212692 20 0.73183
ILMN_1212693 407.6977 0
ILMN_1212695 20 0.76942
ILMN_1212698 20 0.698
ILMN_1212702 20 0.58647
ILMN_1212703 148.15932 0

Total number of rows: 25697

Table truncated, full table size 627 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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