|
| Status |
Public on Apr 22, 2021 |
| Title |
Cell_knockout_day3 |
| Sample type |
RNA |
| |
|
| Source name |
preadipocytes_Irf3-knockout
|
| Organism |
Mus musculus |
| Characteristics |
cell type: preadipocytes
genotype: Irf3-knockout
timepoint: Day 3
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Preadipocytes were cultured with DMEM (high glucose) medium containing 10% NCS plus pen/strep, L-glutamine, 24 nM insulin, and 150 um sodium ascorbate. Medium was changed every two days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from adipocytes at various days during differentiation with TRIzol reagent (Invitrogen)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
KO_D3
|
| Data processing |
Raw data was background subtracted and normalized based on Cross-Correlation method.
The negative and small expression values were set to be 20 based on p value
|
| |
|
| Submission date |
Apr 21, 2021 |
| Last update date |
Apr 22, 2021 |
| Contact name |
Henry Yang |
| E-mail(s) |
csiyangh@nus.edu.sg
|
| Organization name |
Cancer Science Institute of Singapore
|
| Street address |
28 Medical Drive
|
| City |
Singapore |
| ZIP/Postal code |
117456 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE172491 |
Regulation of adipocyte differentiation by IRF3 |
|
