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Sample GSM5257939 Query DataSets for GSM5257939
Status Public on Apr 22, 2021
Title Cell_knockout_day5
Sample type RNA
 
Source name preadipocytes_Irf3-knockout
Organism Mus musculus
Characteristics cell type: preadipocytes
genotype: Irf3-knockout
timepoint: Day 5
Treatment protocol No treatment
Growth protocol Preadipocytes were cultured with DMEM (high glucose) medium containing 10% NCS plus pen/strep, L-glutamine, 24 nM insulin, and 150 um sodium ascorbate. Medium was changed every two days.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from adipocytes at various days during differentiation with TRIzol reagent (Invitrogen)
Label Biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol
Description KO_D5
Data processing Raw data was background subtracted and normalized based on Cross-Correlation method.
The negative and small expression values were set to be 20 based on p value
 
Submission date Apr 21, 2021
Last update date Apr 22, 2021
Contact name Henry Yang
E-mail(s) csiyangh@nus.edu.sg
Organization name Cancer Science Institute of Singapore
Street address 28 Medical Drive
City Singapore
ZIP/Postal code 117456
Country Singapore
 
Platform ID GPL6885
Series (1)
GSE172491 Regulation of adipocyte differentiation by IRF3

Data table header descriptions
ID_REF
VALUE normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212607 20 0.37093
ILMN_1212612 47.621292 0.00376
ILMN_1212619 45.047281 0.00376
ILMN_1212628 20 0.9386
ILMN_1212632 20 0.13409
ILMN_1212636 3894.548 0
ILMN_1212637 1647.4082 0
ILMN_1212645 31.469763 0.03509
ILMN_1212648 353.27448 0
ILMN_1212653 370.06589 0
ILMN_1212672 158.34865 0
ILMN_1212682 98.738109 0
ILMN_1212683 20 0.79699
ILMN_1212685 20 0.19674
ILMN_1212692 20 0.62531
ILMN_1212693 387.2082 0
ILMN_1212695 20 0.37343
ILMN_1212698 20 0.50877
ILMN_1212702 20 0.21303
ILMN_1212703 139.76908 0

Total number of rows: 25697

Table truncated, full table size 623 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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