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Sample GSM5257940 Query DataSets for GSM5257940
Status Public on Apr 22, 2021
Title Cell_knockout_day7
Sample type RNA
 
Source name preadipocytes_Irf3-knockout
Organism Mus musculus
Characteristics cell type: preadipocytes
genotype: Irf3-knockout
timepoint: Day 7
Treatment protocol No treatment
Growth protocol Preadipocytes were cultured with DMEM (high glucose) medium containing 10% NCS plus pen/strep, L-glutamine, 24 nM insulin, and 150 um sodium ascorbate. Medium was changed every two days.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from adipocytes at various days during differentiation with TRIzol reagent (Invitrogen)
Label Biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol
Description KO_D7
Data processing Raw data was background subtracted and normalized based on Cross-Correlation method.
The negative and small expression values were set to be 20 based on p value
 
Submission date Apr 21, 2021
Last update date Apr 22, 2021
Contact name Henry Yang
E-mail(s) csiyangh@nus.edu.sg
Organization name Cancer Science Institute of Singapore
Street address 28 Medical Drive
City Singapore
ZIP/Postal code 117456
Country Singapore
 
Platform ID GPL6885
Series (1)
GSE172491 Regulation of adipocyte differentiation by IRF3

Data table header descriptions
ID_REF
VALUE normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212607 20 0.33835
ILMN_1212612 78.838986 0
ILMN_1212619 29.028154 0.02005
ILMN_1212628 20 0.42481
ILMN_1212632 20 0.27569
ILMN_1212636 3713.6781 0
ILMN_1212637 1434.7645 0
ILMN_1212645 20 0.10276
ILMN_1212648 365.3477 0
ILMN_1212653 342.77261 0
ILMN_1212672 184.24612 0
ILMN_1212682 87.360288 0
ILMN_1212683 20 0.10902
ILMN_1212685 20 0.59023
ILMN_1212692 20 0.14536
ILMN_1212693 297.59304 0
ILMN_1212695 20 0.44612
ILMN_1212698 20 0.46992
ILMN_1212702 20 0.59774
ILMN_1212703 163.37611 0

Total number of rows: 25697

Table truncated, full table size 622 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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