|
Status |
Public on Apr 22, 2021 |
Title |
Cell_knockout_day7 |
Sample type |
RNA |
|
|
Source name |
preadipocytes_Irf3-knockout
|
Organism |
Mus musculus |
Characteristics |
cell type: preadipocytes genotype: Irf3-knockout timepoint: Day 7
|
Treatment protocol |
No treatment
|
Growth protocol |
Preadipocytes were cultured with DMEM (high glucose) medium containing 10% NCS plus pen/strep, L-glutamine, 24 nM insulin, and 150 um sodium ascorbate. Medium was changed every two days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from adipocytes at various days during differentiation with TRIzol reagent (Invitrogen)
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
KO_D7
|
Data processing |
Raw data was background subtracted and normalized based on Cross-Correlation method. The negative and small expression values were set to be 20 based on p value
|
|
|
Submission date |
Apr 21, 2021 |
Last update date |
Apr 22, 2021 |
Contact name |
Henry Yang |
E-mail(s) |
csiyangh@nus.edu.sg
|
Organization name |
Cancer Science Institute of Singapore
|
Street address |
28 Medical Drive
|
City |
Singapore |
ZIP/Postal code |
117456 |
Country |
Singapore |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE172491 |
Regulation of adipocyte differentiation by IRF3 |
|