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Sample GSM5291045 Query DataSets for GSM5291045
Status Public on May 12, 2021
Title E15.5 WT eyes, biological replicate 3
Sample type RNA
 
Source name E15.5 WT Mouse Eyes
Organism Mus musculus
Characteristics sample type: E15.5 mouse eyes
Treatment protocol Eyes from six embryos from each pregnant mouse were isolated and treated as one biological sample. After enucleation, the anterior segments were dissected out and the posterior segments used for RNA extraction
Growth protocol Six pregnant females, three Oa1-/- and three WT, were sacrificed at day 15.5 days post coitum
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Ambion RNA extraction kit
Label Biotin
Label protocol Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 12.5μg of total RNA (Expression Analysis Technical Manual, Affymrtrix)
 
Hybridization protocol Hybridization was performed at 45°C for 16 hours in an Affymetrix Hybridization oven.The arrays were then washed and stained with Streptavidin Phycoerythrin in the Affymetrix Fluidics station (model 450)
Scan protocol Scanning done with the Affymetrix 7G scanner.The Affymetrix GeneChip Command Console 4.0 (AGCC) was used for controlling the system and for acquisition of array images.
Description Gene expression from E15.5 WT mouse Eyes
Data processing Data was processed with Affymetrix MAS5 software, which includes scaling all probes to a target intensity (TGT) of 500.
 
Submission date May 11, 2021
Last update date May 12, 2021
Contact name SONIA GUHA
E-mail(s) guha@jsei.ucla.edu
Organization name UCLA
Department Ophthalmology, SEI
Street address 650 Charles E Young Dr, S
City LOS ANGELES
State/province CA
ZIP/Postal code 90095
Country USA
 
Platform ID GPL1261
Series (1)
GSE174287 Gene Expression Profile From 15 day Embryonic Mouse Eyes

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 428.53 P 5.16732e-05
AFFX-BioB-M_at 772.544 P 4.42873e-05
AFFX-BioB-3_at 460.049 P 4.42873e-05
AFFX-BioC-5_at 1519.47 P 4.42873e-05
AFFX-BioC-3_at 1893.3 P 4.42873e-05
AFFX-BioDn-5_at 3146.02 P 4.42873e-05
AFFX-BioDn-3_at 5538.38 P 7.00668e-05
AFFX-CreX-5_at 19722.6 P 5.16732e-05
AFFX-CreX-3_at 21217.9 P 4.42873e-05
AFFX-DapX-5_at 7.88187 A 0.275146
AFFX-DapX-M_at 11.6733 A 0.354453
AFFX-DapX-3_at 7.61218 A 0.48511
AFFX-LysX-5_at 7.59279 A 0.147939
AFFX-LysX-M_at 9.83423 A 0.659352
AFFX-LysX-3_at 1.5934 A 0.737191
AFFX-PheX-5_at 1.55873 A 0.910522
AFFX-PheX-M_at 1.02176 A 0.988616
AFFX-PheX-3_at 5.45759 A 0.672921
AFFX-ThrX-5_at 3.03068 A 0.724871
AFFX-ThrX-M_at 13.7716 A 0.313723

Total number of rows: 45101

Table truncated, full table size 1396 Kbytes.




Supplementary file Size Download File type/resource
GSM5291045_C-3.CEL.gz 3.7 Mb (ftp)(http) CEL
GSM5291045_C-3.mas5.CHP.gz 389.8 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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