|
| Status |
Public on May 12, 2021 |
| Title |
E15.5 Oa1-/- eyes, Biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
E15.5 Oa1-/- Mouse Eyes
|
| Organism |
Mus musculus |
| Characteristics |
sample type: E15.5 mouse eyes
|
| Treatment protocol |
Eyes from six embryos from each pregnant mouse were isolated and treated as one biological sample. After enucleation, the anterior segments were dissected out and the posterior segments used for RNA extraction
|
| Growth protocol |
Six pregnant females, three Oa1-/- and three WT, were sacrificed at day 15.5 days post coitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Ambion RNA extraction kit
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 12.5μg of total RNA (Expression Analysis Technical Manual, Affymrtrix)
|
| |
|
| Hybridization protocol |
Hybridization was performed at 45°C for 16 hours in an Affymetrix Hybridization oven.The arrays were then washed and stained with Streptavidin Phycoerythrin in the Affymetrix Fluidics station (model 450)
|
| Scan protocol |
Scanning done with the Affymetrix 7G scanner.The Affymetrix GeneChip Command Console 4.0 (AGCC) was used for controlling the system and for acquisition of array images.
|
| Description |
Gene expression from E15.5 Oa1-/- mouse Eyes
|
| Data processing |
Data was processed with Affymetrix MAS5 software, which includes scaling all probes to a target intensity (TGT) of 500.
|
| |
|
| Submission date |
May 11, 2021 |
| Last update date |
May 12, 2021 |
| Contact name |
SONIA GUHA |
| E-mail(s) |
guha@jsei.ucla.edu
|
| Organization name |
UCLA
|
| Department |
Ophthalmology, SEI
|
| Street address |
650 Charles E Young Dr, S
|
| City |
LOS ANGELES |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE174287 |
Gene Expression Profile From 15 day Embryonic Mouse Eyes |
|
