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Sample GSM5291048 Query DataSets for GSM5291048
Status Public on May 12, 2021
Title E15.5 Oa1-/- eyes, Biological replicate 3
Sample type RNA
 
Source name E15.5 Oa1-/- Mouse Eyes
Organism Mus musculus
Characteristics sample type: E15.5 mouse eyes
Treatment protocol Eyes from six embryos from each pregnant mouse were isolated and treated as one biological sample. After enucleation, the anterior segments were dissected out and the posterior segments used for RNA extraction
Growth protocol Six pregnant females, three Oa1-/- and three WT, were sacrificed at day 15.5 days post coitum
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Ambion RNA extraction kit
Label Biotin
Label protocol Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 12.5μg of total RNA (Expression Analysis Technical Manual, Affymrtrix)
 
Hybridization protocol Hybridization was performed at 45°C for 16 hours in an Affymetrix Hybridization oven.The arrays were then washed and stained with Streptavidin Phycoerythrin in the Affymetrix Fluidics station (model 450)
Scan protocol Scanning done with the Affymetrix 7G scanner.The Affymetrix GeneChip Command Console 4.0 (AGCC) was used for controlling the system and for acquisition of array images.
Description Gene expression from E15.5 Oa1-/- mouse Eyes
Data processing Data was processed with Affymetrix MAS5 software, which includes scaling all probes to a target intensity (TGT) of 500.
 
Submission date May 11, 2021
Last update date May 12, 2021
Contact name SONIA GUHA
E-mail(s) guha@jsei.ucla.edu
Organization name UCLA
Department Ophthalmology, SEI
Street address 650 Charles E Young Dr, S
City LOS ANGELES
State/province CA
ZIP/Postal code 90095
Country USA
 
Platform ID GPL1261
Series (1)
GSE174287 Gene Expression Profile From 15 day Embryonic Mouse Eyes

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 363.625 P 9.4506e-05
AFFX-BioB-M_at 578.953 P 4.42873e-05
AFFX-BioB-3_at 341.84 P 5.16732e-05
AFFX-BioC-5_at 1273.62 P 5.16732e-05
AFFX-BioC-3_at 1702.24 P 7.00668e-05
AFFX-BioDn-5_at 2659.09 P 4.42873e-05
AFFX-BioDn-3_at 5090.5 P 8.14279e-05
AFFX-CreX-5_at 17410.3 P 5.16732e-05
AFFX-CreX-3_at 19152.6 P 4.42873e-05
AFFX-DapX-5_at 23.4562 A 0.175328
AFFX-DapX-M_at 20.8604 A 0.250777
AFFX-DapX-3_at 18.1642 A 0.455413
AFFX-LysX-5_at 1.65367 A 0.737191
AFFX-LysX-M_at 3.45631 A 0.772364
AFFX-LysX-3_at 6.5134 A 0.470241
AFFX-PheX-5_at 4.78716 A 0.834139
AFFX-PheX-M_at 1.3054 A 0.949771
AFFX-PheX-3_at 8.27122 A 0.382599
AFFX-ThrX-5_at 39.2703 A 0.108979
AFFX-ThrX-M_at 1.44684 A 0.9273

Total number of rows: 45101

Table truncated, full table size 1393 Kbytes.




Supplementary file Size Download File type/resource
GSM5291048_T-3.CEL.gz 3.8 Mb (ftp)(http) CEL
GSM5291048_T-3.mas5.CHP.gz 389.9 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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